Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor

Sensors
Rosa Helena BustosDiego Jaimes

Abstract

This study describes the development of an immunosensory label-free quantification methodology based on surface plasmon resonance (SPR) and its applicability in measuring/evaluating therapeutic drug monitoring (TDM) of anti-TNF-α monoclonal antibody (adalimumab) in rheumatoid arthritis (RA) patients. The experimental parameters evaluated in this study were immobilising ligands by pre-concentration assays, sensor surface regeneration, ascertaining the method's sensitivity and correlating the results from quantifying plasma samples by ELISA immunoassay. The results showed that TNF-α quantification values (in RU) were significantly different when comparing patients (~50-250 RU) to controls (~10-20 RU). Likewise, there was 0.97 correlation for patients and 0.91 for healthy volunteers using SPR and ELISA comparison methodologies. SPR immunosensory detection provided a precise, sensitive strategy, along with real-time determination, for quantifying adalimumab, having great potential for clinical routine regarding TDM.

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Citations

Nov 14, 2019·Biosensors·Vivian GarzónSandra Bustamante
Feb 6, 2019·Analytical and Bioanalytical Chemistry·Abu Ali Ibn SinaMatt Trau
Nov 25, 2019·Mikrochimica Acta·Farzaneh GhorbaniMehdi Yousefi

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Methods Mentioned

BETA
biosensors
ELISA
biosensor
chip

Software Mentioned

STATA
BIA evaluation
Origin Lab
Originlab

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