Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass

Journal of Proteomics
Sunil S AdavSiu Kwan Sze

Abstract

Solid state fermentation of lignocellulosic biomass by filamentous microorganisms to induced enzyme production has been recognized as an attractive and cost effective technology. The secretion profile of lignocellulolytic enzymes by thermostable filamentous Thermobifida fusca (T. fusca) in solid state fermentation of different lignocellulosic biomasses, such as corn stover, hay; saw dust; sugarcane bagasse; wood chips; and un-dried green plant were explored using label-free exponentially modified protein abundance index (emPAI) based quantitative proteomics. Comparative analyses of T. fusca secretion profiles between cellulose and the various lignocellulosic biomasses showed induced expression of cellulolytic proteins by cellulose, and expression of hemicellulose, pectin and lignin degrading enzymes were induced by lignocellulosic biomasses. The solid state fermentation by T. fusca on lignocellulosic biomasses also revealed increased expressions of various transport proteins and hypothetical proteins. The Bray-Curtis similarity indices, clustering, and multidimensional scaling plot explicated differential protein expressions by T. fusca on different lignocellulosic biomasses, indicating that protein secretion by T. fusca is rel...Continue Reading

References

Jan 1, 1992·Critical Reviews in Biotechnology·D B Wilson
Jul 1, 1974·Canadian Journal of Microbiology·D L Crawford
Mar 14, 1995·Biochemistry·S ZhangD B Wilson
Jan 1, 1993·The Plant Cell·A M Showalter
Jun 13, 2000·Applied Biochemistry and Biotechnology·V S Chang, M T Holtzapple
Jun 7, 2002·Applied Biochemistry and Biotechnology·Donna L WatsonLarry P Walker
Jun 13, 2002·Bioresource Technology·Ye Sun, Jiayang Cheng
Jun 20, 2002·Applied Microbiology and Biotechnology·R GuptaP Lorenz
May 14, 2003·Applied Microbiology and Biotechnology·D IrwinD B Wilson
Jul 9, 2003·European Journal of Biochemistry·Diana C IrwinDavid B Wilson
Apr 1, 1962·Archives of Biochemistry and Biophysics·P ALBERSHEIM, U KILLIAS
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Gladys I. Cassab
May 5, 2006·Applied and Environmental Microbiology·Hideo KawaguchiHideaki Yukawa
May 13, 2006·Biotechnology Advances·Y-H Percival ZhangJonathan R Mielenz
Jan 9, 2007·Journal of Bacteriology·Athanasios LykidisNikos Kyrpides
Mar 21, 2007·Applied and Environmental Microbiology·Yongchao LiDavid B Wilson
Jul 5, 2008·Bioresource Technology·A T W M Hendriks, G Zeeman
Oct 1, 1993·Biotechnology and Bioengineering·D C IrwinD B Wilson
Jul 21, 2009·Journal of Bacteriology·Brian J WatsonSteven W Hutcheson
Apr 23, 2010·Journal of Proteome Research·Sunil S AdavSiu Kwan Sze
Dec 25, 2010·Applied Microbiology and Biotechnology·Anuj K Chandel, Om V Singh
Sep 2, 2011·Journal of Proteome Research·Sunil S AdavSiu Kwan Sze

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Citations

May 10, 2013·PLoS Neglected Tropical Diseases·Kasra Hassani, Martin Olivier
Mar 7, 2014·Journal of Proteome Research·Diana Paola Gómez-MendozaCarlos André Ornelas Ricart
Mar 30, 2013·Critical Reviews in Microbiology·Eva Maria Gomez del Pulgar, Anas Saadeddin
Mar 17, 2015·International Journal of Molecular Sciences·Linda Bianco, Gaetano Perrotta
Dec 3, 2015·Biotechnology for Biofuels·Diego Javier JiménezJan Dirk van Elsas
Sep 8, 2015·Scientific Reports·Diego Javier JiménezJan Dirk van Elsas
Apr 26, 2016·Journal of Proteomics·Tamara MartinovićDjuro Josić
May 14, 2016·Proteomics·Tina Rise TuvengVincent G H Eijsink
Jul 11, 2016·Trends in Biotechnology·Vijai Kumar GuptaRoberto N Silva
Dec 1, 2012·Expert Review of Proteomics·Jean ArmengaudCatherine Duport

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