Label-Free Quantitative Proteomic Analysis of Chitosan Oligosaccharide-Treated Rice Infected with Southern Rice Black-Streaked Dwarf Virus

Viruses
Anming YangBaoan Song

Abstract

Southern rice black-streaked dwarf virus (SRBSDV) has spread from thesouth of China to the north of Vietnam in the past few years and severelyinfluenced rice production. Its long incubation period and early symptoms are not evident; thus, controlling it is difficult. Chitosan oligosaccharide (COS) is a green plant immunomodulator. Early studies showed that preventing and controlling SRBSDV have a certain effect and reduce disease infection rate, but its underlying controlling and preventing mechanism is unclear. In this study, label-free proteomics was used to analyze differentially expressed proteins in rice after COS treatment. The results showed that COS can up-regulate the plant defense-related proteins and down-regulate the protein expression levels of SRBSDV. Meanwhile, quantitative real-time PCR test results showed that COS can improve defense gene expression in rice. Moreover, COS can enhance the defense enzymatic activities of peroxidase, superoxide dismutase and catalase through mitogen-activated protein kinase signaling cascade pathway, and enhance the rice disease resistance.

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Citations

Dec 19, 2018·International Journal of Molecular Sciences·Limin YinBaoan Song
May 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jianke PanDeyu Hu
Jun 30, 2019·International Journal of Biological Macromolecules·Zhurui LiBaoan Song
Jul 3, 2021·Plants·Joanna Kocięcka, Daniel Liberacki
Sep 20, 2019·Journal of Agricultural and Food Chemistry·Dongmei WangBaoan Song
Jul 25, 2019·Journal of Agricultural and Food Chemistry·Fangcheng HeDeyu Hu

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Methods Mentioned

BETA
electrophoresis
acetylation
protein assay
enzyme
PCR
transgenic

Software Mentioned

DAVID
KEGG Automatic Annotation Server
Analyst ( R )
STRING
MaxQuant
Andromeda

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