Label-free Raman spectroscopy for accessing intracellular anticancer drug release on gold nanoparticles

The Analyst
Kwang Su OckSo Yeong Lee

Abstract

We investigated glutathione (GSH)-induced purine or pyrimidine anticancer drug release on gold nanoparticle (AuNP) surfaces by means of label-free Raman spectroscopy. GSH-triggered releases of 6-thioguanine (6TG), gemcitabine (GEM), acycloguanosine (ACY), and fadrozole (FAD) were examined in a comparative way by means of surface-enhanced Raman scattering (SERS). The GSH-induced dissociation constant of GEM (or ACY/FAD) from AuNPs was estimated to be larger by more than 38 times than that of 6TG from the kinetic relationship. Tripeptide control experiments were presented to check the turn-off Raman signalling mechanism. Dark-field microscopy (DFM) and transmission electron microscopy (TEM) indicated the intracellular AuNP loads. After their cellular uptake, GEM, ACY, and FAD would not show SERS intensities as strong as 6TG. This may be due to easier release of GEM, ACY, and FAD than 6TG by intracellular reducing species including GSH. We observed fairly strong SERS signals of GEM and 6TG in cell culture media solution. Our CCK-8 cytotoxicity assay data support that 6TG-AuNPs did not exhibit a substantial decrease in cell viability presumably due to strong binding. Label-free confocal Raman spectroscopy can be utilized as an effe...Continue Reading

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Citations

Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Rizwan WahabAbdulaziz A Al-Khedhairy
Jun 4, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Erdene-Ochir GanboldSang-Woo Joo
Sep 14, 2014·Colloids and Surfaces. B, Biointerfaces·Anh Thu Ngoc LamSang-Woo Joo
Dec 7, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nguyễn Hoàng Ly, Sang-Woo Joo
Nov 14, 2019·Pharmaceutics·Marcin MakowskiNuno C Santos
Jul 28, 2016·Journal of Functional Biomaterials·Li TianMarites P Melancon
Feb 12, 2021·The Science of the Total Environment·Rawil FakhrullinGölnur Fakhrullina

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