Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy

Biomedical Optics Express
Xiang ZhaoWei-Ling Fu

Abstract

The aptamer and target molecule binding reaction has been widely applied for construction of aptasensors, most of which are labeled methods. In contrast, terahertz technology proves to be a label-free sensing tool for biomedical applications. We utilize terahertz absorption spectroscopy and molecular dynamics simulation to investigate the variation of binding-induced collective vibration of hydrogen bond network in a mixed solution of MUC1 peptide and anti-MUC1 aptamer. The results show that binding-induced alterations of hydrogen bond numbers could be sensitively reflected by the variation of terahertz absorption coefficients of the mixed solution in a customized fluidic chip. The minimal detectable concentration is determined as 1 pmol/μL, which is approximately equal to the optimal immobilized concentration of aptasensors.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Feb 1, 1997·Current Opinion in Structural Biology·J W Schwabe
Sep 8, 2001·Journal of Mammary Gland Biology and Neoplasia·S J Gendler
Sep 19, 2002·Analytical Chemistry·Michael LissElke Prohaska
Oct 4, 2005·Journal of Computational Chemistry·David A CaseRobert J Woods
Mar 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jean-Baptiste MassonGuilhem Gallot
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Yaakov Levy, José N Onuchic
Oct 13, 2006·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·C S M FerreiraS Missailidis
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Simon EbbinghausMartina Havenith
Nov 18, 2009·Chemical Reviews·H J Bakker, J L Skinner
Aug 6, 2010·Methods : a Companion to Methods in Enzymology·Matthias Heyden, Martina Havenith
Mar 2, 2011·The Journal of Physical Chemistry. B·Kamila MazurStephen R Meech
Nov 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jessica Dielmann-GessnerIrit Sagi
Dec 9, 2014·Nature Communications·Constantin SchoelerMichael A Nash
May 13, 2015·The Journal of Physical Chemistry. B·Kristen L RhinehardtRam V Mohan
Nov 18, 2015·Journal of Chemical Theory and Computation·Alberto PerezKen A Dill
May 22, 2016·Trends in Biotechnology·Xiang YangYang Luo
Jul 12, 2016·ACS Nano·Hao QuHyongsok Tom Soh
Nov 30, 2016·Biomedical Optics Express·Yan PengYiming Zhu

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