Label-Free Sensors Based on Graphene Field-Effect Transistors for the Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker

Diagnostics
Carrie HaslamShakil A Awan

Abstract

We report on the development of label-free chemical vapour deposition (CVD) graphene field effect transistor (GFET) immunosensors for the sensitive detection of Human Chorionic Gonadotropin (hCG), a glycoprotein risk biomarker of certain cancers. The GFET sensors were fabricated on Si/SiO₂ substrate using photolithography with evaporated chromium and sputtered gold contacts. GFET channels were functionalised with a linker molecule to an immobile anti-hCG antibody on the surface of graphene. The binding reaction of the antibody with varying concentration levels of hCG antigen demonstrated the limit of detection of the GFET sensors to be below 1 pg/mL using four-probe electrical measurements. We also show that annealing can significantly improve the carrier transport properties of GFETs and shift the Dirac point (Fermi level) with reduced p-doping in back-gated measurements. The developed GFET biosensors are generic and could find applications in a broad range of medical diagnostics in addition to cancer, such as neurodegenerative (Alzheimer's and Parkinson's ) and cardiovascular disorders.

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Citations

Nov 5, 2019·Mikrochimica Acta·Leila SyedmoradiKobra Omidfar
Feb 13, 2021·Trends in Biotechnology·Ioannis PrattisLuigi G Occhipinti
Dec 19, 2020·Materials·Patrícia D CabralJérôme Borme
Apr 15, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Tatsuro SaitoYoshiaki Sugizaki
Apr 20, 2021·Frontiers in Molecular Biosciences·Theodore BungonShakil A Awan
May 12, 2021·Scientific Reports·Sotirios PapamatthaiouDespina Moschou

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Methods Mentioned

BETA
biosensing
biosensor
quartz
surface plasmon resonance
biosensors
enzyme-linked immunosorbent assay
ELISA
Atomic Force Microscopy
AFM

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