Label-free signal-on aptasensor for sensitive electrochemical detection of arsenite

Biosensors & Bioelectronics
Lin CuiHuangxian Ju

Abstract

A signal-on aptasensor was fabricated for highly sensitive and selective electrochemical detection of arsenite with a label-free Ars-3 aptamer self-assembled on a screen-printed carbon electrode (SPCE) via Au-S bond. The Ars-3 aptamer could adsorb cationic polydiallyldimethylammonium (PDDA) via electrostatic interaction to repel other cationic species. In the presence of arsenite, the change of Ars-3 conformation due to the formation of Ars-3/arsenite complex led to less adsorption of PDDA, and the complex could adsorb more positively charged [Ru(NH3)6](3+) as an electrochemically active indicator on the aptasensor surface, which produced a sensitive "turn-on" response. The target-induced structure switching could be used for sensitive detection of arsenite with a linear range from 0.2 nM to 100 nM and a detection limit down to 0.15 nM. Benefiting from Ars-3 aptamer, the proposed system exhibited excellent specificity against other heavy metal ions. The SPCE-based aptasensor exhibited the advantages of low cost and simple fabrication, providing potential application of arsenite detection in environment.

Citations

Oct 12, 2016·Biosensors & Bioelectronics·Paria PashazadehMiguel de la Guardia
Oct 18, 2016·Bioconjugate Chemistry·Alejandra TelloHumberto Gomez
Apr 22, 2017·Biosensors & Bioelectronics·Sureshkumar KempahanumakkagariHye-On Yoon
Feb 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei ZhangJun Sheng Lin
Mar 24, 2018·Biosensors·Geetesh Kumar MishraRupesh K Mishra
Nov 2, 2019·Biosensors·Paloma Yáñez-SedeñoJosé Manuel Pingarrón
Jan 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sheng LinDik-Lung Ma
Nov 27, 2020·Advanced Materials·Tedrick Thomas Salim LewMichael S Strano
Feb 12, 2021·International Journal of Nanomedicine·Armin Salek MaghsoudiMohammad Abdollahi
Apr 12, 2021·Talanta·Chen LyuZhouping Wang
Jul 6, 2021·Chemical Communications : Chem Comm·Qi DingHua Kuang

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