Label-free, single molecule resonant cavity detection: a double-blind experimental study

Sensors
Maria V ChistiakovaAndrea M Armani

Abstract

Optical resonant cavity sensors are gaining increasing interest as a potential diagnostic method for a range of applications, including medical prognostics and environmental monitoring. However, the majority of detection demonstrations to date have involved identifying a "known" analyte, and the more rigorous double-blind experiment, in which the experimenter must identify unknown solutions, has yet to be performed. This scenario is more representative of a real-world situation. Therefore, before these devices can truly transition, it is necessary to demonstrate this level of robustness. By combining a recently developed surface chemistry with integrated silica optical sensors, we have performed a double-blind experiment to identify four unknown solutions. The four unknown solutions represented a subset or complete set of four known solutions; as such, there were 256 possible combinations. Based on the single molecule detection signal, we correctly identified all solutions. In addition, as part of this work, we developed noise reduction algorithms.

References

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Citations

Jan 7, 2017·Advanced Materials·Yanyan ZhiYun-Feng Xiao
Nov 15, 2018·Micromachines·Zhiqing Feng, Lan Bai
Sep 17, 2017·Scientific Reports·Tae Young KangKyujung Kim

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Methods Mentioned

BETA
chemical vapor deposition

Software Mentioned

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