Label free targeted detection and quantification of celiac disease immunogenic epitopes by mass spectrometry

Journal of Chromatography. a
Hetty C van den BroeckIngrid M van der Meer

Abstract

Celiac disease (CD) is a food-related disease caused by certain gluten peptides containing T-cell stimulating epitopes from wheat, rye, and barley. CD-patients have to maintain a gluten-free diet and are therefore dependent on reliable testing and labeling of gluten-free products. So far, the R5-ELISA is the approved method to detect if food products can be labeled gluten-free. Because the R5-ELISA detects gluten in general, there is a demand for an improved detection method that quantifies specifically CD-epitopes. Therefore, we developed a new method for detection and quantification of CD-epitopes, based on liquid chromatography (LC) coupled to mass spectrometry (MS) in multiple reaction monitoring (MRM) mode. This method enables targeted label free comparative analysis of the gluten proteins present in different wheat varieties and species, and in wheat-based food products. We have tested our method by analyzing several wheat varieties that vary in CD-epitope content, as was shown before using immunoblotting and specific monoclonal antibodies. The results showed that a modern bread wheat variety Toronto contained the highest amounts of CD immunogenic peptides compared with the older bread wheat variety Minaret and the tetrap...Continue Reading

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Citations

Mar 21, 2016·Journal of Proteomics·María José Martínez-EstesoGavin O'Connor
Jul 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandrina ShatalovaYuri Lebedin
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Mar 13, 2020·Frontiers in Plant Science·Susan B AltenbachArmin Alaedini
May 18, 2020·International Journal of Molecular Sciences·Olha LakhnekoĽudovit Škultéty
Dec 5, 2019·Frontiers in Plant Science·Thais O AlvesMariana S L Ferreira
Aug 18, 2021·Comprehensive Reviews in Food Science and Food Safety·Miguel RibeiroGilberto Igrejas

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