Label-free visualization of fruit lignification: Raman molecular imaging of loquat lignified cells

Plant Methods
Nan ZhuKunsong Chen

Abstract

Flesh lignification, leading to increased fruit firmness, has been reported in several kinds of fruit. Understanding the mechanisms underlying fruit lignification is important to optimize the postharvest storage strategies and reduce the quality deterioration of postharvest fruit. Especially cellular level investigation of lignin deposition in fruits provides novel insight for deciphering the mechanisms underlying fruit lignification. The primary objective of this study was to establish a procedure of using Raman microspectroscopy technique to depict fruit lignification at the cell level. Lignified cells, a special kind of cells contained high lignin content, were found abundantly scattered in red-fleshed 'Luoyangqing' loquat. Whereas these special lignified cells were barely detected in 'Baisha' loquat flesh. Dominant Raman bands of lignified cells were found primarily attributed to lignin (1664, 1628, 1603, 1467, and 1272 cm-1), cellulose (1383, 1124 and 1098 cm-1) and pectin (852 and 1740 cm-1). The band intensity correlation analysis indicated the peak at 1335 cm-1 assigned to either lignin or cellulose in previous works was related to lignin for the lignified cells. Multi-peaks Gaussian fitting successfully resolved the ov...Continue Reading

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Citations

Feb 9, 2021·Frontiers in Plant Science·William Z Payne, Dmitry Kurouski
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aneta SaletnikCzesław Puchalski

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Methods Mentioned

BETA
imaging techniques
Raman
confocal microscopy
Raman microscopy
imaging technique
Light microscopy

Software Mentioned

Origin

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