Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles

Journal of Biophotonics
Greggy M SantosWei-Chuan Shih

Abstract

We experimentally demonstrate a label-free biosensor for the ERBB2 cancer gene DNA target based on the distance-dependent detection of surface-enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non-porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays. With patterned nanoporous gold disk (NPGD) plasmonic nanoparticles, a label-free biosensor that makes use of distance-dependent detection of surface-enhanced fluorescence (SEF) is constructed and tested for zeptomole detection of ERBB2 cancer gene DNA targets.

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Citations

Jun 1, 2016·Journal of the American Chemical Society·Pallavi DaggumatiErkin Seker
Aug 28, 2016·Journal of Colloid and Interface Science·Zaneta Swiatkowska-WarkockaKenji Kawaguchi
Nov 1, 2016·ACS Applied Materials & Interfaces·Suyan QiuWei-Chuan Shih
Oct 11, 2017·Nanomedicine·Md Masud Parvez Arnob, Wei-Chuan Shih
Oct 31, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Leo T H de JeerJeff Th M De Hosson
Jul 1, 2017·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Suyan QiuWei-Chuan Shih
Sep 1, 2017·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Fusheng ZhaoWei-Chuan Shih

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