Labeling and localization of the herpes simplex virus capsid protein UL25 and its interaction with the two triplexes closest to the penton.

Journal of Molecular Biology
J F ConwayF L Homa

Abstract

The herpes simplex virus type 1 UL25 protein is one of seven viral proteins that are required for DNA cleavage and packaging. Together with UL17, UL25 forms part of an elongated molecule referred to as the C-capsid-specific component (CCSC). Five copies of the CCSC are located at each of the capsid vertices on DNA-containing capsids. To study the conformation of UL25 as it is folded on the capsid surface, we identified the sequence recognized by a UL25-specific monoclonal antibody and localized the epitope on the capsid surface by immunogold electron microscopy. The epitope mapped to amino acids 99-111 adjacent to the region of the protein (amino acids 1-50) that is required for capsid binding. In addition, cryo-EM reconstructions of C-capsids in which the green fluorescent protein (GFP) was fused within the N-terminus of UL25 localized the point of contact between UL25 and GFP. The result confirmed the modeled location of the UL25 protein in the CCSC density as the region that is distal to the penton with the N-terminus of UL25 making contact with the triplex one removed from the penton. Immunofluorescence experiments at early times during infection demonstrated that UL25-GFP was present on capsids located within the cytoplasm...Continue Reading

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Citations

Aug 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kui Yang, Joel D Baines
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kevin Patrick BohannonGregory Allan Smith
Sep 2, 2011·Journal of Virology·Mindy LeelawongGregory A Smith
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Sep 24, 2015·Viruses·Danielle J OwenStephen C Graham
Jul 24, 2014·Nucleic Acids Research·Udom Sae-UengAlex Evilevitch
Nov 28, 2015·Viruses·Ian B HogueLynn W Enquist
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Jun 15, 2012·Virology·William W NewcombJay C Brown
Oct 17, 2017·The Journal of General Virology·Yun-Tao LiuZ Hong Zhou
Jun 15, 2018·Journal of Virology·Gina R DanielGregory A Smith
Mar 10, 2017·Journal of Virology·Nabil El BilaliRoger Lippé
May 27, 2016·Journal of Virology·Bernadette M DeRussyRitesh Tandon
Oct 30, 2016·Journal of Virology·Oana MaierGregory A Smith
Apr 26, 2016·Nature Structural & Molecular Biology·Alexis HuetJames F Conway
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson
Sep 25, 2020·Journal of Virology·Alexis HuetFred L Homa

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