Labeling glycans on living cells by a chemoenzymatic glycoengineering approach

Biology Open
Ruben T Almaraz, Yanhong Li

Abstract

Structural glycobiology has traditionally been a challenging field due to a limited set of tools available to investigate the diverse and complex glycan molecules. However, we cannot ignore that glycans play critical roles in health as well as in disease, and are present in more than 50% of all proteins and on over 80% of all surface proteins. Chemoenzymatic glycoengineering (CGE) methods are a powerful set of tools to synthesize complex glycans, but the full potential of these methods have not been explored in cell biology yet. Herein, we report the labeling of live Chinese hamster ovary (CHO) cells by employing three highly specific glycosyltransferases: a sialyltransferase, a galactosyltransferase, and an N-acetyl-glucosaminyl transferase. We verified our results by bio-orthogonal blots and further rationalized them by computational modeling. We expect CGE applications in cell biology to rise and their implementation will assist in structural-functional discoveries in glycobiology. This research will contribute to this effort.

References

Apr 30, 2005·Current Opinion in Plant Biology·Dianna BowlesBrigitte Poppenberger
Nov 23, 2006·Methods in Enzymology·Santosh Kumar Patnaik, Pamela Stanley
Jun 6, 2009·Glycobiology·Patrick HosslerZheng Jian Li
Apr 8, 2011·Angewandte Chemie·Tianqing ZhengPeng Wu
Sep 6, 2013·Journal of Industrial Microbiology & Biotechnology·Nguyen Huy Thuan, Jae Kyung Sohng
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Feb 11, 2016·Biochemical Society Transactions·John B McArthur, Xi Chen
Apr 14, 2016·Glycobiology·Nicole Nischan, Jennifer J Kohler

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Citations

Jan 18, 2018·Chemistry : a European Journal·Xiaobao BiChuan-Fa Liu

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Methods Mentioned

BETA
glycosylation

Software Mentioned

SCIGRESS
Scigress FJ

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