Labeling of pancreatic islets with iron oxide nanoparticles for in vivo detection with magnetic resonance

Transplantation
Zuzana BerkováFrantisek Saudek

Abstract

In vitro labeling of pancreatic islets with iron nanoparticles enables their direct posttransplant visualization by magnetic resonance; however, there is still a discrepancy in the fate of iron nanoparticles. This study was performed to detail the labeling process, consequently to improve the labeling efficacy and to confirm safety for islet cells. The islets were visible on T2*-weighted magnetic resonance images as hypointense spots immediately after 1-hr cultivation. Although at this time already the sufficient superparamagnetic effect was achieved, most of the particles were deposed in islet macrophages and only later were they found in endosomes of endocrine islet cells. The iron content depended on length of culture period. The labeled islets showed an intact ultrastructure, responded normally to glucose stimulation in vitro, and were able to treat experimental diabetes. For purpose of subsequent magnetic resonance imaging, a 24-hr culture with ferucarbotran leads to sufficient labeling with no apparent adverse effect on beta cell morphology or function.

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Citations

Dec 17, 2009·Topics in Magnetic Resonance Imaging : TMRI·Juliana Puppi, Michel Modo
Jan 5, 2011·Transplantation·Frantisek SaudekMilan Hájek
Oct 21, 2011·Journal of Transplantation·Cyrus JahansouzKenneth L Brayman
Jun 13, 2008·The Review of Diabetic Studies : RDS·Frantisek SaudekSrirang Manohar
Jan 1, 2009·Nanoscale Research Letters·Zheng WangDaxiang Cui
Aug 18, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M L MalosioA Del Maschio
Oct 28, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yang JiaoCui-Ping Zhong
Feb 4, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J H KimK-W Kim
Dec 23, 2011·Transplant International : Official Journal of the European Society for Organ Transplantation·J KrizF Saudek
Sep 20, 2012·Contrast Media & Molecular Imaging·Klára ZacharovováFrantišek Saudek
Oct 18, 2016·Topics in Magnetic Resonance Imaging : TMRI·Ashley V MakelaPaula J Foster
Mar 15, 2018·Journal of Personalized Medicine·Rita Sofia Garcia RibeiroUwe Himmelreich
Jan 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yongqiang DongWenqing Liang

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