Labelling of lymphocytes with indium 111 oxine: effect on cell surface phenotype and antibody-dependent cellular cytotoxicity

Immunology Letters
A SignoreP Pozzilli

Abstract

Indium 111 oxine is currently used to label peripheral lymphocytes in order to study the kinetics of these cells in vivo. Since the quantity of radioisotope for labelling is still a matter of controversy, we have investigated in vitro the effect of increasing the concentration of indium 111 oxine on the lymphocyte surface phenotype and the antibody-dependent cellular cytotoxicity (ADCC) using lymphocytes from normal subjects. The cell surface phenotype, as evaluated by 2 monoclonal antibodies, was not affected whereas ADCC, at any of the doses used, was significantly reduced compared to the baseline value. The implications of these results for the use of indium 111 oxine for the in vivo studies are discussed.

References

Jul 1, 1983·Clinical Endocrinology·P PozzilliA G Cudworth

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Citations

Mar 20, 2012·Circulation Research·Kirsti A CampbellColeen A McNamara
Aug 28, 2002·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Alberto SignoreFrancesco Scopinaro
Apr 19, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Alessio AnnovazziAlberto Signore
Mar 4, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Manel RocaAlberto Signore
Jul 1, 1984·Seminars in Nuclear Medicine·J P Dutcher
Apr 1, 1989·Immunological Reviews·J W Kupiec-Weglinski, N L Tilney
Mar 2, 2006·International Journal of Environmental Health Research·Hong-Liang LiuXue-Min Chen
Dec 1, 2018·Contrast Media & Molecular Imaging·S AulettaA Signore
Apr 9, 1999·Journal of Endocrinological Investigation·A SignoreR Barone
Aug 15, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Filippo GalliAlberto Signore

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