PMID: 7539321Feb 1, 1995Paper

Labelling of polysaccharides using [11C]cyanogen bromide. In vivo and in vitro evaluation of 11C-hyaluronan uptake kinetics

Nuclear Medicine and Biology
G WesterbergB Långström

Abstract

A method for the 11C-labelling of polysaccharides in high specific radioactivity is described. Dextran and hyaluronan were treated with [11C]cyanogen bromide in aqueous solution at pH 11.5 to give 30-47% radiochemical yields with higher than 98% radiochemical purity in synthesis times of 24-26 min counted from the end of bombardment. Specific radioactivities at the end of synthesis ranged from 0.12 to 3.1 Ci/mumol. The biodistribution kinetics of [11C]hyaluronan injected intravenously was studied in rats by means of positron emission tomography, showing a rapid and displaceable uptake in liver. Uptake and displacement of [11C]hyaluronan was also demonstrated in cultured rat liver endothelial cells.

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Citations

Nov 15, 2008·European Journal of Drug Metabolism and Pharmacokinetics·E SvanovskyM Laznicek
Jul 25, 2000·European Journal of Clinical Investigation·U LindqvistB Långström
Feb 3, 2006·Biopolymers·Petra MlcochováMartin Krcmár
Jun 5, 2012·Pharmacological Reports : PR·Milan LaznicekVladimir Velebny
Nov 8, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Damien JamesEric Fouquet
Mar 2, 2010·Journal of Pharmaceutical and Biomedical Analysis·D CozikovaV Velebny

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