PMID: 6539126May 30, 1984Paper

Labelling of the human erythrocyte glucose transporter with 3H-labelled cytochalasin B occurs via protein photoactivation

Biochimica Et Biophysica Acta
M DezielA Klip

Abstract

Irradiation of human erythrocyte membranes with 3H-labelled cytochalasin B results in specific photolabelling of the glucose transporter. The action spectrum of photolabelling has a maximum at approx. 280 nm, whereas the absorption spectrum of cytochalasin B is maximal at 210 nm. By irradiating with narrow-band-width light centered at 280 nm for 2 h, 8% of the transporters become covalently labelled and 47% of the remaining cytochalasin B-binding sites are obliterated. We conclude that photolabelling driven by narrow-bandwidth irradiation proceeds via photoactivation of an aromatic amino acid residue on the transporter molecule, and when compared to wide-bandwidth irradiation, permits more efficient incorporation of the label without causing additional photodamage to the remaining transporters.

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Citations

Aug 1, 1990·Journal of Bioenergetics and Biomembranes·P J Henderson
Jan 1, 1987·Pharmacology & Therapeutics·S M Jarvis, J D Young
Mar 1, 1990·Research in Microbiology·P J Henderson
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A P DickD M Walker
Oct 1, 1992·The Journal of Clinical Investigation·V SarabiaA Klip
May 1, 1988·Journal of Cellular Physiology·J M May
Oct 17, 1989·Biochemistry·A Carruthers, A L Helgerson
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·L F BissonD A Lewis
Dec 1, 1988·Annals of Neurology·R N KalariaS I Harik
Dec 1, 1988·Journal of Neurochemistry·S I HarikR N Kalaria
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Henderson, M C Maiden
Aug 18, 1988·Biochimica Et Biophysica Acta·J M May, B J Danzo
Jun 18, 1991·Biochimica Et Biophysica Acta·A K Englund, P Lundahl
Apr 21, 1992·Journal of Theoretical Biology·J C Butte

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