Abstract
Labile iron pool (LIP) is intracellular nonprotein bound iron that can generate oxygen radicals via the Fenton reaction resulting in oxidative cell damage. Therefore, quantitative measurement of LIP will be helpful for detecting and monitoring the toxic iron status in iron overloaded patients. This study demonstrated LIP level and its correlation to oxidative stress status in β-thalassemic erythrocytes. LIP and reactive oxygen species (ROS) level, numbers of erythrocyte vesicles and apoptosis were assayed by flow cytometric methods in 30 blood samples from β-thalassemia/hemoglobin E patients and 17 blood samples from healthy volunteers with normal hemoglobin type. β-thalassemic erythrocytes showed higher LIP level, defined as the difference in calcein fluorescent intensity of the cells treated with or without deferiprone, than normal erythrocytes (mean ± 2SD as 62.39 ± 39.58 versus 44.65 ± 35.86, P = 0.003). The LIP level above 67, a cutoff value of LIP level obtained from receiver operating characteristic curve analysis, had a significant positive correlation with oxidative stress status for ROS level (r = 0.90, P < 0.001) and also the amount of erythrocyte vesicles (r = 0.79, P = 0.002). In contrast, the LIP level showed a si...Continue Reading
References
Jun 1, 1993·Baillière's Clinical Haematology·E Shinar, E A Rachmilewitz
Jan 24, 2002·Journal of Hepatology·Giuliana ZanninelliZ Ioav Cabantchik
Oct 11, 2002·Free Radical Biology & Medicine·Or Kakhlon, Z Ioav Cabantchik
Feb 13, 2003·European Journal of Haematology·Johnny AmerEitan Fibach
Dec 30, 2003·Cytometry. Part B, Clinical Cytometry·Kovit PattanapanyasatH Kyle Webster
May 25, 2004·Blood·Pensri PootrakulZ Ioav Cabantchik
Dec 13, 2005·Annals of the New York Academy of Sciences·Eliezer A RachmilewitzZ Ioav Cabantchik
May 28, 2008·British Journal of Haematology·Eugenia Prus, Eitan Fibach
Jul 3, 2008·International Journal of Hematology·Yutaka KohgoJunji Kato
Jul 16, 2008·Biochimica Et Biophysica Acta·Inna FreikmanEitan Fibach
Jun 30, 2009·The Annals of Thoracic Surgery·Michael HaaseAnja Haase-Fielitz
Aug 23, 2011·Biochimica Et Biophysica Acta·Pierre BrissotOlivier Loréal
Aug 7, 2013·Nutrients·Andrea U Steinbicker, Martina U Muckenthaler
Citations
Nov 6, 2018·Metallomics : Integrated Biometal Science·Matthias MisslingerHubertus Haas
Apr 24, 2019·Journal of Clinical Pathology·Pornthip ChaichompooSuchin Worawichawong
Aug 9, 2020·Metallomics : Integrated Biometal Science·Daniel J Kosman
Dec 29, 2020·Frontiers in Cellular Neuroscience·Paul J DerryThomas A Kent
May 8, 2020·Leukemia Research·Jiaxin HuLijuanli Li
Mar 18, 2021·Scientific Reports·Usama AbbasiJayachandran N Kizhakkedathu
Jul 31, 2021·Life Sciences·Samir A Salama, Mostafa M Elshafey
Feb 15, 2019·Bioconjugate Chemistry·Shreya PramanikDibakar Goswami
Aug 24, 2020·Chemico-biological Interactions·Samir A Salama, Ahmed M Kabel