Labile iron pool as a parameter to monitor iron overload and oxidative stress status in β-thalassemic erythrocytes

Cytometry. Part B, Clinical Cytometry
Boonyanuch ChutvanichkulWanchai Wanachiwanawin

Abstract

Labile iron pool (LIP) is intracellular nonprotein bound iron that can generate oxygen radicals via the Fenton reaction resulting in oxidative cell damage. Therefore, quantitative measurement of LIP will be helpful for detecting and monitoring the toxic iron status in iron overloaded patients. This study demonstrated LIP level and its correlation to oxidative stress status in β-thalassemic erythrocytes. LIP and reactive oxygen species (ROS) level, numbers of erythrocyte vesicles and apoptosis were assayed by flow cytometric methods in 30 blood samples from β-thalassemia/hemoglobin E patients and 17 blood samples from healthy volunteers with normal hemoglobin type. β-thalassemic erythrocytes showed higher LIP level, defined as the difference in calcein fluorescent intensity of the cells treated with or without deferiprone, than normal erythrocytes (mean ± 2SD as 62.39 ± 39.58 versus 44.65 ± 35.86, P = 0.003). The LIP level above 67, a cutoff value of LIP level obtained from receiver operating characteristic curve analysis, had a significant positive correlation with oxidative stress status for ROS level (r = 0.90, P < 0.001) and also the amount of erythrocyte vesicles (r = 0.79, P = 0.002). In contrast, the LIP level showed a si...Continue Reading

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Citations

Nov 6, 2018·Metallomics : Integrated Biometal Science·Matthias MisslingerHubertus Haas
Apr 24, 2019·Journal of Clinical Pathology·Pornthip ChaichompooSuchin Worawichawong
Aug 9, 2020·Metallomics : Integrated Biometal Science·Daniel J Kosman
Feb 15, 2019·Bioconjugate Chemistry·Shreya PramanikDibakar Goswami

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