Laboratory evaluation of the Liat HIV Quant (IQuum) whole-blood and plasma HIV-1 viral load assays for point-of-care testing in South Africa

Journal of Clinical Microbiology
Lesley ScottWendy Stevens

Abstract

Point-of-care (POC) HIV viral load (VL) testing offers the potential to reduce turnaround times for antiretroviral therapy monitoring, offer near-patient acute HIV diagnosis in adults, extend existing centralized VL services, screen women in labor, and prompt pediatrics to early treatment. The Liat HIV Quant plasma and whole-blood assays, prerelease version, were evaluated in South Africa. The precision, accuracy, linearity, and agreement of the Liat HIV Quant whole-blood and plasma assays were compared to those of reference technologies (Roche CAP CTMv2.0 and Abbott RealTime HIV-1) on an HIV verification plasma panel (n = 42) and HIV clinical specimens (n = 163). HIV Quant plasma assay showed good performance, with a 2.7% similarity coefficient of variation (CV) compared to the Abbott assay and a 1.8% similarity CV compared to the Roche test on the verification panel, and 100% specificity. HIV Quant plasma had substantial agreement (pc [concordance correlation] = 0.96) with Roche on clinical specimens and increased variability (pc = 0.73) in the range of <3.0 log copies/ml range with the HIV Quant whole-blood assay. HIV Quant plasma assay had good linearity (2.0 to 5.0 log copies/ml; R(2) = 0.99). Clinical sensitivity at a vir...Continue Reading

References

Jun 1, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·C DelamareC Rouzioux
Jan 16, 1999·Journal of Clinical Microbiology·H H KesslerE Marth
Aug 31, 2002·AIDS Research and Human Retroviruses·Maria A PapathanasopoulosFrancine E McCutchan
Jun 27, 2003·Cytometry. Part B, Clinical Cytometry·Lesley E ScottDeborah K Glencross
Dec 5, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alexandra CalmyWilliam Rodriguez
Dec 7, 2007·Pediatrics·Jennifer S Read, UNKNOWN Committee on Pediatric AIDS, American Academy of Pediatrics
Mar 18, 2008·The Journal of Antimicrobial Chemotherapy·Ae S YoungpairojJ Gerardo García-Lerma
Aug 12, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Asgeir JohannessenCarmen de Mendoza
Sep 25, 2009·The Journal of Antimicrobial Chemotherapy·Asgeir JohannessenAlexandra Calmy
Mar 17, 2010·The Journal of Infectious Diseases·Wendy S StevensSuzanne M Crowe
Mar 17, 2010·The Journal of Infectious Diseases·Sultan TanriverdiShuqi Chen
Apr 24, 2010·Emergency Medicine Clinics of North America·Wesley H Self
Sep 21, 2012·Journal of Clinical Microbiology·Lesley E ScottWendy Stevens
Jul 18, 2014·Journal of Clinical Microbiology·Allyson V RitchieHelen H Lee

❮ Previous
Next ❯

Citations

Feb 16, 2016·Viruses·Dana S ClutterRobert W Shafer
Jun 8, 2017·AIDS·Patrick Jean-PhilippeElaine J Abrams
Apr 16, 2018·Epidemiology and Infection·A HahnH Frickmann
Jun 30, 2018·Sexually Transmitted Infections·Gary Brook
Nov 1, 2016·Journal of Clinical Microbiology·Sikhulile MoyoVladimir Novitsky
Nov 30, 2018·Clinical Microbiology Reviews·Bharat S ParekhJohn N Nkengasong
May 17, 2019·Clinical Microbiology Reviews·Paul K DrainNigel Garrett
Oct 23, 2018·Medicina·Sello Lebohang ManotoPatience Mthunzi-Kufa
Feb 25, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Junpeng ZhaoLunan Wang
Jul 12, 2017·Journal of the International AIDS Society·Sarah E RutsteinJoseph D Tucker
Dec 21, 2016·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·Thomas MeyerNorbert H Brockmeyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.