Laboratory evaluation on the sensitivity and specificity of a novel and rapid detection method for malaria diagnosis based on magneto-optical technology (MOT).

Malaria Journal
Petra F MensHenk D F H Schallig

Abstract

This study describes the laboratory evaluation of a novel diagnostic platform for malaria. The Magneto Optical Test (MOT) is based on the bio-physical detection of haemozoin in clinical samples. Having an assay time of around one minute, it offers the potential of high throughput screening. Blood samples of confirmed malaria patients from different regions of Africa, patients with other diseases and healthy non-endemic controls were used in the present study. The samples were analysed with two reference tests, i.e. an histidine rich protein-2 based rapid diagnostic test (RDT) and a conventional Pan-Plasmodium PCR, and the MOT as index test. Data were entered in 2 x 2 tables and analysed for sensitivity and specificity. The agreement between microscopy, RDT and PCR and the MOT assay was determined by calculating Kappa values with a 95% confidence interval. The observed sensitivity/specificity of the MOT test in comparison with clinical description, RDT or PCR ranged from 77.2 - 78.8% (sensitivity) and from 72.5 - 74.6% (specificity). In general, the agreement between MOT and the other assays is around 0.5 indicating a moderate agreement between the reference and the index test. However, when RDT and PCR are compared to each othe...Continue Reading

References

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Citations

Aug 14, 2013·Trends in Parasitology·Márcia BouraThomas Hänscheid
Nov 4, 2011·The American Journal of Tropical Medicine and Hygiene·Stephan KarlTim G St Pierre
Feb 18, 2014·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Marceli KoralewskiJacek Gierszewski
Dec 26, 2015·Trends in Parasitology·Thomas HänscheidMartin P Grobusch
Dec 19, 2014·The Cochrane Database of Systematic Reviews·Katharine AbbaYemisi Takwoingi
Apr 17, 2015·Malaria Journal·Maria RebeloThomas Hänscheid
Jul 24, 2015·Current Opinion in Infectious Diseases·Peter A Zimmerman, Rosalind E Howes
Oct 1, 2014·Clinical Chemistry·Molly Webster, Vikram Sheel Kumar
Aug 18, 2016·Expert Review of Anti-infective Therapy·Brian T Grimberg, Kerry O Grimberg
Feb 14, 2021·Nature Communications·L ArndtS Karl
Feb 9, 2018·ACS Applied Materials & Interfaces·Marceli KoralewskiPeter Kopčanský
Dec 5, 2018·Chemical Reviews·Christine F MarkwalterDavid W Wright

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

Dyn
MOT
Cell

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