Laboratory evolution for forced glucose-xylose co-consumption enables identification of mutations that improve mixed-sugar fermentation by xylose-fermenting Saccharomyces cerevisiae

FEMS Yeast Research
Ioannis PapapetridisJack T Pronk

Abstract

Simultaneous fermentation of glucose and xylose can contribute to improved productivity and robustness of yeast-based processes for bioethanol production from lignocellulosic hydrolysates. This study explores a novel laboratory evolution strategy for identifying mutations that contribute to simultaneous utilisation of these sugars in batch cultures of Saccharomyces cerevisiae. To force simultaneous utilisation of xylose and glucose, the genes encoding glucose-6-phosphate isomerase (PGI1) and ribulose-5-phosphate epimerase (RPE1) were deleted in a xylose-isomerase-based xylose-fermenting strain with a modified oxidative pentose-phosphate pathway. Laboratory evolution of this strain in serial batch cultures on glucose-xylose mixtures yielded mutants that rapidly co-consumed the two sugars. Whole-genome sequencing of evolved strains identified mutations in HXK2, RSP5 and GAL83, whose introduction into a non-evolved xylose-fermenting S. cerevisiae strain improved co-consumption of xylose and glucose under aerobic and anaerobic conditions. Combined deletion of HXK2 and introduction of a GAL83G673T allele yielded a strain with a 2.5-fold higher xylose and glucose co-consumption ratio than its xylose-fermenting parental strain. These ...Continue Reading

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Citations

Nov 16, 2019·World Journal of Microbiology & Biotechnology·Yusuf SürmeliZeynep Petek Çakar
Jun 1, 2020·Applied Microbiology and Biotechnology·Luís C MartinsIsabel Sá-Correia
Sep 25, 2020·Applied Microbiology and Biotechnology·Yanfeng LiuLong Liu
Feb 7, 2019·Frontiers in Microbiology·Meirong GaoZengyi Shao
Sep 13, 2019·Current Opinion in Biotechnology·Nian LiuGregory Stephanopoulos
Oct 4, 2020·Trends in Biotechnology·Gregory GuirimandVincent Courdavault
Apr 8, 2021·FEMS Yeast Research·Carla Inês Soares RodriguesAndreas K Gombert
Jul 12, 2021·Biotechnology Advances·Maria MavrommatiGeorge Aggelis
Aug 4, 2021·Nature Reviews. Microbiology·José Montaño LópezJosé L Avalos
May 18, 2019·Journal of Agricultural and Food Chemistry·Ronivaldo Rodrigues da SilvaEleni Gomes

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Methods Mentioned

BETA
PCR
genetic modifications
ubiquitination

Software Mentioned

MATLAB
SBML Toolbox
Gurobi
libSBML

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