Laboratory Growth and Genetic Manipulation of Eimeria tenella

Current Protocols in Microbiology
Iván Pastor-FernándezVirginia Marugán-Hernández

Abstract

Eimeria is a genus of apicomplexan parasites that contains a large number of species, most of which are absolutely host-specific. Seven species have been recognized to infect chickens. Infection of susceptible chickens results in an intestinal disease called coccidiosis, characterized by mucoid or hemorrhagic enteritis, which is associated with impaired feed conversion or mortality in severe cases. Intensive farming practices have increased the significance of coccidiosis since parasite transmission is favored by high-density housing of large numbers of susceptible chickens. Routine chemoprophylaxis and/or vaccination with live parasite vaccines provides effective control of Eimeria, although the emergence of drug resistance and the relative cost and production capacity of current vaccine lines can prove limiting. As pressure to reduce drug use in livestock production intensifies, novel vaccination strategies are needed. Development of effective protocols supporting genetic complementation of Eimeria species has until recently been hampered by their inability to replicate efficiently in vitro. Now, the availability of such protocols has raised the prospect of generating transgenic parasite lines that function as vaccine vectors...Continue Reading

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Citations

Feb 26, 2020·Infection and Immunity·Xinming TangXun Suo
Aug 28, 2020·Animals : an Open Access Journal From MDPI·Xu SongZhongqiong Yin
Apr 8, 2021·Avian Pathology : Journal of the W.V.P.A·Damer P BlakeFiona M Tomley
Oct 12, 2021·Frontiers in Genetics·Peipei ChengFeng Gu

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