Laboratory investigations of Hb Constant Spring

Clinical and Laboratory Haematology
O TangvarasittichaiTorpong Sanguansermsri


Hb Constant Spring (HbCS), a nondeletional alpha-thalassemia, is most prevalent in southern Chinese and southeast Asian populations. In conjunction with alpha-thalassemia-1 or in the homozygous state, it is an important cause of HbH disease. The present study was designed to test the efficiency of different diagnostic methods in detecting HbCS. The following laboratory tests were applied to blood samples from 1000 pregnant women attending an antenatal clinic at Buddhachinaraj Hospital in Phitsanulok, Thailand: mutation specific restriction enzyme digestion (RED), amplification refractory mutation system (ARMS) and automated high-performance liquid chromatography (HPLC). The results demonstrate that the DNA-based methods, RED and ARMS, are efficient diagnostic tools in detecting HbCS in homozygotes and heterozygotes, whereas automated HPLC gave accurate results for homozygous HbCS, but misidentified (no peak) some cases of HbCS trait. Standard hematological methods (determination of mean cell hemoglobin and mean cell volume) did not efficiently differentiate homozygous HbCS and HbCS trait from samples with normal alpha-globin chains. We conclude that RED is the preferable method for HbCS screening and then confirmed diagnostic t...Continue Reading


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Related Concepts

Hemoglobin Constant Spring
High Pressure Liquid Chromatography Procedure
DNA, Double-Stranded
Abnormal Hemoglobins
Restriction Fragment Length Polymorphism
Restriction Mapping
Laboratory Diagnosis
Nucleic Acid Amplification Tests

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