Laboratory maintenance of Bdellovibrio

Current Protocols in Microbiology
Carey Lambert, R Elizabeth Sockett

Abstract

Bdellovibrio bacteriovorus is a Gram-negative bacterium that preys upon other Gram-negative bacteria. It does this by penetrating the outer membrane and peptidoglycan to establish itself in the periplasm where it grows at the expense of the contents of the prey cell before ultimately lysing the prey. Wild-type Bdellovibrio are prey-(or host-) dependent and the protocols described in this unit deal with the techniques required to grow this bacterium on its prey cells. Protocols are also presented to generate and culture host-independent mutants as well as novel protocols to evaluate Bdellovibrio predation.

References

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Dec 1, 1986·Molecular & General Genetics : MGG·M RogersD Sherratt
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Citations

Jan 12, 2010·PloS One·Carey LambertR Elizabeth Sockett
Sep 23, 2014·Applied and Environmental Microbiology·Megan A FergusonEileen M Spain
Jun 28, 2011·Applied and Environmental Microbiology·Robert J AtterburyR Elizabeth Sockett
May 17, 2017·Current Protocols in Microbiology·Mélanie J BoileauKenneth D Clinkenbeard
Jun 13, 2009·FEMS Microbiology Reviews·James E Berleman, John R Kirby
May 12, 2019·Scientific Reports·James S FindlayPetra C F Oyston
Jun 26, 2018·M. Auxiliadora PrietoVirginia Martínez

Related Concepts

Bacteriological Techniques
Bdellovibrio
Alkalescens-Dispar Group
Cryonic Suspension
Gram-Negative Bacteria
Peptidoglycan
Evaluation
Bdellovibrio bacteriovorus
Mutant
Outer Membrane

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