Laboratory Mice Are Frequently Colonized with Staphylococcus aureus and Mount a Systemic Immune Response-Note of Caution for In vivo Infection Experiments

Frontiers in Cellular and Infection Microbiology
Daniel SchulzSilva Holtfreter

Abstract

Whether mice are an appropriate model for S. aureus infection and vaccination studies is a matter of debate, because they are not considered as natural hosts of S. aureus. We previously identified a mouse-adapted S. aureus strain, which caused infections in laboratory mice. This raised the question whether laboratory mice are commonly colonized with S. aureus and whether this might impact on infection experiments. Publicly available health reports from commercial vendors revealed that S. aureus colonization is rather frequent, with rates as high as 21% among specific-pathogen-free mice. In animal facilities, S. aureus was readily transmitted from parents to offspring, which became persistently colonized. Among 99 murine S. aureus isolates from Charles River Laboratories half belonged to the lineage CC88 (54.5%), followed by CC15, CC5, CC188, and CC8. A comparison of human and murine S. aureus isolates revealed features of host adaptation. In detail, murine strains lacked hlb-converting phages and superantigen-encoding mobile genetic elements, and were frequently ampicillin-sensitive. Moreover, murine CC88 isolates coagulated mouse plasma faster than human CC88 isolates. Importantly, S. aureus colonization clearly primed the mur...Continue Reading

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Citations

May 29, 2020·BMC Musculoskeletal Disorders·Lars HelbigHolger Freischmidt
Dec 15, 2018·The Journal of Infectious Diseases·Xinhai ChenOlaf Schneewind
Oct 1, 2017·Scientific Reports·Amy FlaxmanDavid H Wyllie
Feb 2, 2021·Frontiers in Immunology·Silke MachataIlse D Jacobsen
Dec 10, 2020·Microorganisms·Erin I ArmentroutGislâine A Martins
Oct 1, 2020·International Journal of Molecular Sciences·Daniel M MrochenSilva Holtfreter

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Methods Mentioned

BETA
gene knock-out
genotyping
PCR
PCRs
electrophoresis
IEC
FCS
ELISA

Software Mentioned

xPONENT
R
SpaServer
GraphPadPrismX3
Envigo
R Development Core Team
Ridom Software
FlexMap®

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