Laboratory quality control in a multicentre meningococcal carriage study in Burkina Faso

Transactions of the Royal Society of Tropical Medicine and Hygiene
Paul A KristiansenDominique A Caugant

Abstract

To investigate the potential herd immunity effect of MenAfriVac, a new conjugate vaccine against serogroup A Neisseria meningitidis, a multiple cross-sectional carriage study was conducted in three districts in Burkina Faso in 2009, yielding a total of 20 326 oropharyngeal samples. A major challenge was the harmonisation of operational procedures and ensuring the reliability of results. Here we describe the laboratory quality control (QC) system that was implemented. Laboratory analysis performed by three local laboratories included colony morphology assessment, oxidase test, Gram stain, β-galactosidase activity using o-nitrophenyl-β-galactopyranoside (ONPG), γ-glutamyl transferase (GGT) activity and slide agglutination serogrouping. Internal QC was performed on media, reagents, laboratory equipment and field conditions. Confirmation of results and molecular characterisation was performed at the Norwegian Institute of Public Health (Oslo, Norway). External QC was performed on 3% of specimens where no colonies morphologically resembling N. meningitidis had been identified and on 10% of non-ONPG-/GGT+ isolates. The QC system was a critical element: it identified logistical and operational problems in real time and ensured accurac...Continue Reading

References

Aug 1, 1988·Epidemiology and Infection·J V PetherS C Sheard
Jun 5, 2002·Lancet·Martin C J MaidenUNKNOWN UK Meningococcal Carraige Group
Dec 3, 2003·Tropical Medicine & International Health : TM & IH·S DjiboJ-P Chippaux
Aug 18, 2004·Journal of Medical Microbiology·Siamak P Yazdankhah, Dominique A Caugant
May 19, 2006·Emerging Infectious Diseases·Jenny MacLennanUNKNOWN United Kingdom Meningococcal Carriage Group
Jan 20, 2007·FEMS Microbiology Reviews·Dominique A CaugantPaula Kriz
Nov 30, 2007·The Lancet Infectious Diseases·Caroline L Trotter, Brian M Greenwood
Jan 27, 2009·The Journal of Infection·Jonathan RobertsJames Stuart
Jan 14, 2011·Clinical and Vaccine Immunology : CVI·Paul A KristiansenDominique A Caugant

❮ Previous
Next ❯

Citations

Oct 23, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Paul A KristiansenDominique A Caugant
Oct 23, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Martin C J Maiden
Oct 5, 2012·Journal of Clinical Microbiology·Paul A KristiansenDominique A Caugant
May 21, 2013·Tropical Medicine & International Health : TM & IH·UNKNOWN MenAfriCar Consortium
Nov 22, 2018·Tropical Medicine & International Health : TM & IH·Brian M GreenwoodCaroline L Trotter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Meningitis

Bacterial meningitis continues to be an important cause of mortality and morbidity throughout the world. Here is the latest research.

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Bacterial Meningitis (ASM)

Bacterial meningitis continues to be an important cause of mortality and morbidity throughout the world. Here is the latest research.

Related Papers

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Paul A KristiansenDominique A Caugant
Transactions of the Royal Society of Tropical Medicine and Hygiene
Amina Amadou HamidouSuzanne Chanteau
© 2022 Meta ULC. All rights reserved