Laboratory Studies of Nonlinear Optical Signals for Caries Detection

Journal of Dental Research
E TerrerT Cloitre

Abstract

Multiphoton confocal microscopy and nonlinear spectroscopy are used to investigate the caries process in dentin. Although dentin is a major calcified tissue of the teeth, its organic phase comprises type I collagen fibers. Caries drive dentin demineralization and collagen denaturation. Multiphoton microscopy is a powerful imaging technique: the biological materials are transparent to infrared frequencies and can be excited to penetration depths inaccessible to 1-photon confocal microscopy. The laser excitation greatly reduces photodamage to the sole focal region, and the signal-to-noise ratio is improved significantly. The method has been used to follow pathologic processes involving collagen fibrosis or collagen destruction based on their 2-photon excited fluorescence (2PEF) emission and second harmonic generation (SHG). Combining multiphoton imaging with nonlinear spectroscopy, we demonstrate that both 2PEF and SHG intensity of human dentin are strongly modified during the tooth caries process, and we show that the ratio between SHG and 2PEF signals is a reliable parameter to follow dental caries. The ratio of the SHG/2PEF signals measured by nonlinear optical spectroscopy provides valuable information on the caries process, ...Continue Reading

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Citations

Aug 16, 2016·Journal of Dental Research·Y MatsudaT Araki
Mar 7, 2018·Caries Research·Amel SlimaniElodie Terrer
Sep 9, 2018·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Fabio Martins SalomãoTiciane Cestari Fagundes
Feb 15, 2019·Lasers in Medical Science·Livia Tosi TrevelinAdriana Bona Matos
Jul 18, 2019·Journal of Dental Research·D G MoussaC Aparicio

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Methods Mentioned

BETA
imaging technique
light scattering

Software Mentioned

WinSpec
Image J

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