Laccase immobilization on the electrode surface to design a biosensor for the detection of phenolic compound such as catechol

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Maryam NazariRonak Rafipour

Abstract

Biosensors based on the coupling of a biological entity with a suitable transducer offer an effective route to detect phenolic compounds. Phenol and phenolic compounds are among the most toxic environmental pollutants. Laccases are multi-copper oxidases that can oxide phenol and phenolic compounds. A method is described for construction of an electrochemical biosensor to detect phenolic compounds based on covalent immobilization of laccase (Lac) onto polyaniline (PANI) electrodeposited onto a glassy carbon (GC) electrode via glutaraldehyde coupling. The modified electrode was characterized by voltammetry, Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) techniques. The results indicated that laccase was immobilized onto modified GC electrode by the covalent interaction between laccase and terminal functional groups of the glutaraldehyde. The laccase immobilized modified electrode showed a direct electron transfer reaction between laccase and the electrode. Linear range, sensitivity, and detection limit for this biosensor were 3.2 × 10(-6) to 19.6 × 10(-6)M, 706.7 mAL mol(-1), 2.07 × 10(-6)M, respectively.

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Citations

Sep 1, 2015·Biosensors & Bioelectronics·Ioana VasilescuGabriel-Lucian Radu
Dec 17, 2016·Biotechnology and Applied Biochemistry·Maria Lepore, Marianna Portaccio
Mar 29, 2019·Biotechnology and Applied Biochemistry·Nasim MalekiNahid Shahabadi
Sep 22, 2020·Critical Reviews in Analytical Chemistry·Nurgul K BakirhanSibel A Ozkan
Jan 17, 2020·Sensors·Sylwia BalutaJoanna Cabaj
May 24, 2020·Polymers·Kamila SpychalskaJoanna Cabaj
Feb 1, 2020·International Journal of Biological Macromolecules·Congqiang LouZhanhu Guo
Nov 17, 2020·Analytica Chimica Acta·Paulo A Raymundo-PereiraOrlando Fatibello-Filho
Dec 31, 2020·Polymers·Simonas Ramanavicius, Arunas Ramanavicius
Nov 2, 2020·International Journal of Biological Macromolecules·Muhammad BilalHafiz M N Iqbal
Feb 9, 2019·International Journal of Biological Macromolecules·Changiz Karami, Mohammad Ali Taher
Aug 10, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Adriany das Graças Nascimento AmorimBeatriz López-Ruiz
Aug 20, 2021·Environmental Technology·Wyllerson Evaristo GomesRenata Kelly Mendes
Oct 15, 2020·Chemical Reviews·Hui ChenShelley D Minteer

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