Laccase isoenzymes of Pleurotus eryngii: characterization, catalytic properties, and participation in activation of molecular oxygen and Mn2+ oxidation.

Applied and Environmental Microbiology
C MuñozM J Martínez

Abstract

Two laccase isoenzymes produced by Pleurotus eryngii were purified to electrophoretic homogeneity (42- and 43-fold) with an overall yield of 56.3%. Laccases I and II from this fungus are monomeric glycoproteins with 7 and 1% carbohydrate content, molecular masses (by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) of 65 and 61 kDa, and pIs of 4.1 and 4.2, respectively. The highest rate of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) oxidation for laccase I was reached at 65 degrees C and pH 4, and that for laccase II was reached at 55 degrees C and pH 3.5. Both isoenzymes are stable at high pH, retaining 60 to 70% activity after 24 h from pH 8 to 12. Their amino acid compositions and N-terminal sequences were determined, the latter strongly differing from those of laccases of other basidiomycetes. Antibodies against laccase I reacted with laccase II, as well as with laccases from Pleurotus ostreatus, Pleurotus pulmonarius, and Pleurotus floridanus. Different hydroxy- and methoxy-substituted phenols and aromatic amines were oxidized by the two laccase isoenzymes from P. eryngii, and the influence of the nature, number, and disposition of aromatic-ring substituents on kinetic constants is discussed. Although both...Continue Reading

References

Jul 1, 1991·Journal of General Microbiology·M SaloheimoJ K Knowles
Jan 1, 1990·Chemico-biological Interactions·K OllingerE Cadenas
Aug 28, 1990·European Journal of Biochemistry·J J BonoJ L Seris
Jul 15, 1988·The Biochemical Journal·P M HannaB Reinhammar
Jan 1, 1987·Annual Review of Microbiology·T K Kirk, R L Farrell
Oct 1, 1974·Journal of Bacteriology·S C Froehner, K E Eriksson
Jun 1, 1981·Archives of Biochemistry and Biophysics·C C Winterbourn
Apr 1, 1982·Archives of Biochemistry and Biophysics·F S Archibald, I Fridovich
Mar 1, 1995·Applied and Environmental Microbiology·Y Fukushima, T K Kirk
Jun 1, 1995·Applied and Environmental Microbiology·P GiardinaG Sannia
Jun 1, 1993·Journal of General Microbiology·C R PerryC F Thurston
Apr 15, 1996·European Journal of Biochemistry·M J MartínezA T Martínez
Feb 1, 1996·European Journal of Biochemistry·P GiardinaG Sannia
Jan 1, 1952·The Biochemical Journal·W E TREVELYAN, J S HARRISON
Oct 1, 1984·Applied and Environmental Microbiology·J M Bollag, A Leonowicz
Aug 1, 1994·Applied and Environmental Microbiology·F Guillén, C S Evans
Dec 1, 1995·Applied and Environmental Microbiology·C SrinivasanC A Reddy

❮ Previous
Next ❯

Citations

Jan 5, 2000·Applied and Environmental Microbiology·F GuillénM Jesús Martínez
Oct 30, 2001·FEMS Microbiology Letters·F SolanoA Sanchez-Amat
Apr 4, 2002·Molecular Microbiology·Alexander SchoutenJan A L van Kan
Dec 9, 2003·Applied and Environmental Microbiology·Tania GonzálezAldo E González
Jul 26, 2005·Biochemistry. Biokhimii︠a︡·D A CadimalievV D Samuilov
Feb 23, 2008·Organic & Biomolecular Chemistry·Mahelet Aweke TadesseFederica Sergi
Oct 24, 2008·Phytopathology·Ian A ThompsonDarrell G Schulze
Jun 12, 2009·Critical Reviews in Biotechnology·Mirjana StajićSonja Duletić-Lausević
Jan 1, 2004·FEMS Microbiology Ecology·Naoyuki MiyataMitsuyuki Soma
Feb 2, 2013·Iranian Journal of Environmental Health Science & Engineering·Sidra Ilyas, Abdul Rehman
Jan 1, 2006·Plant Disease·V V LozovayaG L Hartman
Mar 28, 2002·Applied and Environmental Microbiology·Mario C N SaparratMaría Jesús Martínez
Feb 7, 2004·Biotechnology Progress·C LópezJ M Lema
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·O V MorozovaA I Yaropolov
Apr 21, 2009·Applied and Environmental Microbiology·Víctor Gómez-ToribioFrancisco Guillén
Apr 26, 2014·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Bea-Ven ChangHung-Yu Liao
Mar 8, 2014·Environmental Technology·Bea-Ven ChangChien-Sen Liao
Apr 5, 2003·Applied and Environmental Microbiology·M Enriqueta AriasManuel Hernández
Aug 3, 2005·Applied Microbiology and Biotechnology·H X Wang, T B Ng
Jun 17, 1999·FEBS Letters·C Höfer, D Schlosser
Dec 10, 2002·Archives of Microbiology·Teresa de la RubiaJosé Martínez
Nov 7, 2008·Applied Microbiology and Biotechnology·Jong-Rok JeonYoon-Seok Chang
Aug 11, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·R BériaultV D Appanna
Apr 21, 2009·Applied and Environmental Microbiology·Víctor Gómez-ToribioFrancisco Guillén
Apr 28, 2011·Applied Microbiology and Biotechnology·Patrizia Di GennaroGuido Sello
Mar 11, 2011·Bioscience, Biotechnology, and Biochemistry·Thitinard NitheranontYasuhiko Asada
Jan 18, 2012·Applied Microbiology and Biotechnology·Aitor Hernández-OrtegaAngel T Martínez
Jan 24, 2013·Antonie van Leeuwenhoek·Martin RühlUrsula Kües
Apr 11, 2009·Biotechnology Letters·Kristiina HildénTaina Lundell
Sep 22, 2012·Protein Engineering, Design & Selection : PEDS·Gianluca BleveFrancesco Grieco
Apr 16, 2013·Preparative Biochemistry & Biotechnology·Amado I Grandes-BlancoMartha D Bibbins-Martínez
Mar 31, 2009·Folia Microbiologica·J M BarrasaM J Martínez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.