Laccase-Mediated Enhancement of the Antioxidant Activity of Propolis and Poplar Bud Exudates

ACS Omega
Lorenzo BottaRaffaele Saladino

Abstract

The treatment of propolis and poplar bud exudates with laccase from Trametes versicolor and 2,2,6,6-tetramethyl-1-piperidinyloxy free radical increased the antioxidant activity, as evaluated by the 2,2'-diphenyl picrylhydrazyl (DPPH)- and t-butyl-OOH-induced DNA breakage comet assay analyses. The effect was highest for shorter reaction times. Propolis showed the highest antioxidant activity in the DPPH test, whereas poplar bud exudates were more active in reducing the t-butyl-OOH-induced lesions in the Chinese hamster ovary cell line. Even if the concentration of polyphenols decreased during the oxidation, the formation of low-molecular-weight phenols phloroglucinol 4 (1,3,5-trihydroxy benzene), hydroquinone 5 (1,4-dihydroxy benzene), and catechol 6 (1,2-dihydroxy benzene), characterized by the radical-scavenging activity, can account for the observed increase in the antioxidant activity.

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Citations

Nov 9, 2018·Applied Microbiology and Biotechnology·Abha SharmaR C Kuhad
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Nov 28, 2018·Environmental Science and Pollution Research International·Pingjing WenQijun Wu

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Methods Mentioned

BETA
NMR
Assay
Electrophoresis

Software Mentioned

Varian
GraphPad Prism
MS Data Review

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