Abstract
DNA topoisomerases are major defined targets for a large variety of clinically important anticancer agents, including etoposide, adriamycin, and mitoxantrone. Mutations at amino acids 439, 450 and 803 of DNA topoisomerase II were examined in multiple anticancer drug-resistant anaplastic thyroid carcinomas (ten cell lines and three cancerous tissues) by reverse transcriptase-polymerase chain reaction (RT-PCR) and subsequent DNA sequencing. No mutation was found in these cell lines and tissues, but mdr1, mrp and/or lrp mRNA were expressed to a varying degree, and there was no significant difference in DNA topoisomerase IIalpha content among the cell lines and tissues as evaluated by Western blotting. Our experimental data indicate that overexpression of multidrug resistance-related mRNA is sufficient to confer drug resistance.
References
Aug 1, 1992·Japanese Journal of Cancer Research : Gann·I SugawaraS Itoyama
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B Y BuggD P Suttle
Sep 1, 1991·Japanese Journal of Cancer Research : Gann·I SugawaraS Mori
Dec 17, 1989·Biochimica Et Biophysica Acta·P D'Arpa, L F Liu
Dec 1, 1986·The Journal of Cell Biology·M M Heck, W C Earnshaw
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B C GuildD E Housman
Jan 1, 1985·Mayo Clinic Proceedings·C J NelC S Grant
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Aug 16, 1995·Cancer Letters·I SugawaraT Yamashita
Sep 1, 1994·Somatic Cell and Molecular Genetics·T SumizawaS Akiyama
Jan 15, 1994·Cancer·M ItsuboM Tanaka
Jul 29, 1994·Cancer Letters·I SugawaraS Itoyama
Jan 1, 1993·Cancer Chemotherapy and Pharmacology·Y Pommier
Jan 15, 1997·Cancer Letters·I SugawaraS Itoyama
Citations
Sep 16, 1998·Thyroid : Official Journal of the American Thyroid Association·K B Ain
Aug 25, 2000·Thyroid : Official Journal of the American Thyroid Association·K B AinP A DeSimone
Mar 7, 2009·Thyroid : Official Journal of the American Thyroid Association·Colin J MooneyScot C Remick
Jul 21, 2011·Journal of Oncology·Govardhanan NagaiahScot C Remick
Feb 14, 2006·Annals of Surgical Oncology·Chandrakanth Are, Ashok R Shaha
Apr 22, 2008·Annales d'endocrinologie·J-L Wemeau, C Do Cao
Apr 6, 2001·Baillière's Best Practice & Research. Clinical Endocrinology & Metabolism·K B Ain
Feb 16, 2005·Anti-cancer Drugs·C MassartJ Gibassier
Jun 27, 2002·British Journal of Cancer·J TennvallG Wallin
Apr 29, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·A LeeZ W Baloch
Nov 1, 2011·Expert Review of Endocrinology & Metabolism·Ejigayehu G Abate, Robert C Smallridge
Jul 5, 2019·Endocrinology·Elnaz AbbasifaridVahid Haghpanah
Jan 16, 1999·Seminars in Surgical Oncology·K B Ain
Jan 22, 2005·Scandinavian Journal of Surgery : SJS : Official Organ for the Finnish Surgical Society and the Scandinavian Surgical Society·G WallinJ Tennvall