Lack of a vacuolar sorting receptor leads to non-specific missorting of soluble vacuolar proteins in Arabidopsis seeds

Traffic
Christian P CraddockLorenzo Frigerio

Abstract

The plant vacuolar sorting receptor (VSR) binds proteins carrying vacuolar sorting signals (VSS) of the 'sequence-specific' type (ssVSS) but not the C-terminal, hydrophobic sorting signals (ctVSS). Seeds of Arabidopsis mutants lacking the major VSR isoform, AtVSR1, secrete a proportion of the proteins destined to storage vacuoles. The sorting signals for these proteins are not well defined, but they do not seem to be of the ssVSS type. Here, we tested whether absence of VSR1 in seeds leads to secretion of reporter proteins carrying ssVSS but not ctVSS. Our results show that reporters carrying either ssVSS or ctVSS are equally secreted in the absence of VSR1. We discuss our findings in relation to the current model for vacuolar sorting.

References

Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·T KirschJ C Rogers
Nov 22, 1997·The Plant Cell·E PedrazziniA Vitale
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
May 14, 1999·Trends in Plant Science·A Vitale, N V Raikhel
Apr 8, 2003·Plant Molecular Biology·Nicholas A JolliffeLynne M Roberts
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Keito NishizawaShigeru Utsumi
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Joanna C BrownLynne M Roberts
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tomoo ShimadaIkuko Hara-Nishimura
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Svetlana EpimashkoGerhard Thiel
Feb 12, 2004·The Plant Journal : for Cell and Molecular Biology·Nicole HappelSusanne E H Holstein
Sep 3, 2004·The Plant Journal : for Cell and Molecular Biology·Nicholas A JolliffeLorenzo Frigerio
Dec 1, 2004·Traffic·Dirk WenzelGiselbert Hinz
Jun 14, 2005·Trends in Plant Science·Alessandro Vitale, Giselbert Hinz
Jul 7, 2005·Traffic·David G RobinsonGiselbert Hinz
Aug 24, 2005·The Journal of Cell Biology·Misoon ParkInhwan Hwang
Jun 6, 2006·Phytochemical Analysis : PCA·Simona Birtić, Ilse Kranner
Jun 30, 2006·Current Opinion in Cell Biology·Barbara BorgonovoMichele Solimena

❮ Previous
Next ❯

Citations

Aug 6, 2008·Plant Physiology·Enrique Rojo, Jurgen Denecke
Oct 8, 2008·Plant Physiology·Inhwan Hwang
Apr 15, 2010·Plant Physiology·Elsa ArcalisEva Stoger
Jan 12, 2010·The Plant Cell·Abel RosadoNatasha V Raikhel
May 7, 2009·Traffic·Sandra RichterGerd Jürgens
Jun 10, 2008·Traffic·Lorenzo FrigerioDavid G Robinson
Jul 17, 2008·Traffic·Ombretta Foresti, Jürgen Denecke
Dec 18, 2012·The FEBS Journal·Li XiangWim Van den Ende
Jun 7, 2013·The Plant Journal : for Cell and Molecular Biology·Jinbo ShenLiwen Jiang
Dec 25, 2009·The Plant Journal : for Cell and Molecular Biology·Andrea PompaMichele Bellucci
Nov 15, 2011·American Journal of Botany·Brendon F ConlanMarilyn A Anderson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.