Mar 25, 2020

Practical and effective diagnosis of animal anthrax in endemic low-resource settings

BioRxiv : the Preprint Server for Biology
Olubunmi Rhoda AminuT. Forde

Abstract

BackgroundAnthrax threatens human and animal health, and peoples livelihoods in many rural communities in Africa and Asia. In these areas, anthrax surveillance is challenged by a lack of tools for on-site detection. Furthermore, cultural practices and infrastructure may affect sample availability and quality. Practical yet accurate diagnostic solutions are greatly needed to quantify anthrax impacts. We validated microscopic and molecular methods for the detection of Bacillus anthracis in field-collected blood smears and identified alternative samples suitable for anthrax confirmation in the absence of blood smears. Methodology/Principal FindingsWe investigated livestock mortalities suspected to be caused by anthrax in northern Tanzania. Field-prepared blood smears (n = 152) were tested by microscopy using four staining techniques as well as polymerase chain reaction (PCR) followed by Bayesian latent class analysis. Median sensitivity (91%, CI 95% [84-96%]) and specificity (99%, CI 95% [96-100%]) of microscopy using azure B were comparable to those of the recommended standard, polychrome methylene blue, PMB (92%, CI 95% [84-97%] and 98%, CI 95% [95-100%], respectively), but azure B is more available and convenient. Other common...Continue Reading

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Mentioned in this Paper

Gene Polymorphism
Study
TLR4 gene
IGHE
Lung
Genes
Toll-like receptor 4
Enzymes, antithrombotic
Research Subject
Chronic Disease

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