PMID: 8943041Nov 26, 1996Paper

Lack of chromosome territoriality in yeast: promiscuous rejoining of broken chromosome ends

Proceedings of the National Academy of Sciences of the United States of America
James E Haber, W Y Leung

Abstract

Various studies suggest that eukarytoic chromosomes may occupy distinct territories within the nucleus and that chromosomes are tethered to a nuclear matrix. These constraints might limit interchromosomal interactions. We have used a molecular genetic test to investigate whether the chromosomes of Saccharomyces cerevisiae exhibit such territoriality. A chromosomal double-strand break (DSB) can be efficiently repaired by recombination between flanking homologous repeated sequences. We have constructed a strain in which DSBs are delivered simultaneously to both chromosome III and chromosome V by induction of the HO endonuclease. The arrangement of partially duplicated HIS4 and URA3 sequences around each HO recognition site allows the repair of the two DSBs in two alternative ways: (i) the creation of two intrachromosomal deletions or (ii) the formation of a pair of reciprocal translocations. We show that reciprocal translocations are formed approximately as often as the pair of intrachromosomal deletions. Similar results were obtained when one of the target regions was moved from chromosome V to any of three different locations on chromosome XI. These results argue that the broken ends of mitotic chromosomes are free to search th...Continue Reading

References

Apr 1, 1992·Current Opinion in Genetics & Development·U K LaemmliY Adachi
Feb 1, 1992·Molecular Microbiology·M Roberge, S M Gasser
Jan 1, 1991·Methods in Cell Biology·D Carroll, C W Lehman
Jun 1, 1991·Current Opinion in Cell Biology·S M Gasser
Dec 14, 1990·Science·L Manuelidis
Jun 5, 1989·Journal of Molecular Biology·J A NickoloffF Heffron
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·R E Jensen, I Herskowitz
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·T CremerP Lichter
Jul 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J E Haber
May 1, 1994·The Journal of Cell Biology·V GuacciD Koshland
Jul 1, 1993·Molecular and Cellular Biology·S Jinks-RobertsonS Ramcharan
Feb 1, 1993·The Journal of Cell Biology·Y HiraokaJ W Sedat

❮ Previous
Next ❯

Citations

Jan 29, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Leonid A Mirny
Aug 1, 2000·Mutation Research·J E Haber
Aug 1, 2000·Mutation Research·P SungS Van Komen
Nov 1, 2000·Nature Cell Biology·L Aragón-Alcaide, A V Strunnikov
May 4, 2010·Nature·Zhijun DuanWilliam S Noble
May 9, 2007·Nature Cell Biology·Evi SoutoglouTom Misteli
Nov 4, 2008·Nature Methods·Axel B BergerChristophe Zimmer
Nov 2, 2005·Nature Reviews. Microbiology·Michael M Cox, John R Battista
Mar 27, 1998·Molecular Microbiology·K Carlson, L D Kosturko
Jun 24, 2006·DNA and Cell Biology·Matthew J Pratt-HyattDavid R Engelke
Apr 17, 2009·Molecular Biology of the Cell·Alan Michael Tartakoff, Purnima Jaiswal
Apr 27, 2013·Journal of Biochemistry·Hajime KimuraTakashi Ohyama
Feb 16, 2006·Nucleic Acids Research·Haruna TsukiokaTakahiro Kusakabe
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·C Richardson, M Jasin
Oct 16, 2004·Molecular and Cellular Biology·Bertrand Llorente, Lorraine S Symington
Apr 4, 2006·Molecular and Cellular Biology·Ezra SchildkrautJac A Nickoloff
Aug 30, 2008·PLoS Computational Biology·Angelo Rosa, Ralf Everaers
Nov 13, 2012·PLoS Genetics·Diana D VillarrealSang Eun Lee
Apr 1, 2010·Epigenomics·Shigeki NagaiSusan M Gasser
Dec 15, 2010·International Journal of Biological Sciences·Jun DaiWei Hu
Apr 3, 2014·Nature Cell Biology·Vassilis Roukos, Tom Misteli
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·A S Goldman, M Lichten
Oct 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael M Vilenchik, Alfred G Knudson
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·W LeungJ E Haber
Jan 10, 2012·Annual Review of Immunology·Beth A Helmink, Barry P Sleckman
Mar 9, 2011·The Journal of Cell Biology·Christophe Zimmer, Emmanuelle Fabre
Dec 3, 2014·Journal of Molecular Biology·Charlène Lemaître, Evi Soutoglou
Jan 23, 2016·Mutation Research·Supriya SinhaSang Eun Lee
Oct 27, 2011·Seminars in Cell & Developmental Biology·Torben R Kasparek, Timothy C Humphrey
Jul 25, 2009·Fungal Genetics and Biology : FG & B·C D M RodleyJ M O'Sullivan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.