PMID: 6166581Jan 15, 1981Paper

Lack of correlation between in vivo and in vitro assays for the detection of virus released from clones of Friend erythroleukemia cells

International Journal of Cancer. Journal International Du Cancer
L BertoliniC Friend

Abstract

The biological properties of the virus synthesized by 18 clones of a line of mouse bone-marrow hematopoietic cells transformed in vitro by the polycythemic strain of Friend leukemia virus (FLV-P) were compared. In vitro assays were performed to determine whether the virus released into the culture fluids was ecotropic or xenotropic, and in vivo assays were carried out to determine spleen focus formation and leukemogenicity in susceptible DBA/2J and BALB/c mice. A number of clones released virus which reproduced the entire range of effects typical of the FLY-P complex. However, in other clones, there appeared to be no correlation between the assays for leukemogenicity and the assays for either ecotropic virus, reverse transcriptase activity, or virus antigens. Xenotropic virus was not detected in any of the cultures. These results suggest that the FLV-P complex contains a heterogeneous population of viruses, but the possibility that the differences observed may be due to the inability of FLV-P to be expressed fully in some clones cannot be excluded.

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R RevoltellaC Friend
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J W HartleyW P Rowe
Jun 1, 1979·Journal of Cellular Physiology·R P RevoltellaG Torlontano
Jan 1, 1979·Leukemia Research·P E TambourinF Smadja-Joffe
Jan 1, 1977·Journal of Virology·W SchäferD P Bolognesi
Dec 1, 1970·Virology·W P RoweJ W Hartley
Jan 15, 1970·International Journal of Cancer. Journal International Du Cancer·K E Rowson, I B Parr
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J A BilelloJ T August
Feb 1, 1973·The Journal of Experimental Medicine·F G Gudat, W Villiger
Feb 19, 1971·Nature·R H BassinP J Fischinger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.