Lack of coupling between secondary structure formation and collapse in a model polypeptide that mimics early folding intermediates, the F2 fragment of the Escherichia coli tryptophan-synthase beta chain

Protein Science : a Publication of the Protein Society
K GastM E Goldberg

Abstract

The isolated, 101-residue long C-terminal (so called F2) fragment of the beta chain from Escherichia coli tryptophan synthase was shown previously to fold into an ensemble of conformations that are condensed, to contain large amounts of highly dynamic secondary structures, and to behave as a good model of structured intermediates that form at the very early stages of protein folding. Here, solvent perturbations were used to investigate the forces that are involved in stabilizing the secondary structure (monitored by far-UV CD) and the condensation of the polypeptide chain (monitored by dynamic light scattering) in isolated F2. It was observed that neither the ionic strength, nor the pH (between 7 and 10), nor salts of the Hofmeister series affected the global secondary structure contents of F2, whereas some of these salts affected the collapse slightly. Addition of trifluoroethanol resulted in a large increase in both the amount of secondary structure and the Stokes radius of F2. Conversely, F2 became more condensed upon raising the temperature from 4 to 60 degrees C, whereas in this temperature range, the secondary structure undergoes significant melting. These observations lead to the conclusion that, in isolated F2, there is...Continue Reading

References

Feb 1, 1975·FEBS Letters·O RaibaudM E Goldberg
Jan 1, 1992·European Biophysics Journal : EBJ·K GastD Zirwer
Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·J M Scholtz, R L Baldwin
May 5, 1991·Journal of Molecular Biology·L M Gregoret, F E Cohen
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H S Chan, K A Dill
Feb 20, 1989·Journal of Molecular Biology·P L PrivalovN N Khechinashvili
Jan 1, 1972·Progress in Biophysics and Molecular Biology·A A Zamyatnin
Mar 3, 1981·Biochemistry·J P Hennessey, W C Johnson
Apr 1, 1995·Protein Science : a Publication of the Protein Society·K A DillH S Chan
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·A L Fink
Apr 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·O B PtitsynV N Uversky
Mar 17, 1995·Science·P G WolynesD Thirumalai
May 24, 1994·Biochemistry·E E LattmanK A Dill
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C M JonesW A Eaton
Feb 13, 1993·Biochimica Et Biophysica Acta·G DamaschunD Behnke
Oct 1, 1996·Biophysical Journal·R L Baldwin
Oct 15, 1996·Structure·W A EatonJ Hofrichter
Jan 1, 1981·Biopolymers·José M García Bernal, José García De La Torre

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