Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes I and III

Biochimica Et Biophysica Acta
Elina WelchenHans-Peter Braun

Abstract

We studied the role of cytochrome c (CYTc), which mediates electron transfer between Complexes III and IV, in cellular events related with mitochondrial respiration, plant development and redox homeostasis. We analyzed single and double homozygous mutants in both CYTc-encoding genes from Arabidopsis: CYTC-1 and CYTC-2. While individual mutants were similar to wild-type, knock-out of both genes produced an arrest of embryo development, showing that CYTc function is essential at early stages of plant development. Mutants in which CYTc levels were extremely reduced respective to wild-type had smaller rosettes with a pronounced decrease in parenchymatic cell size and an overall delay in development. Mitochondria from these mutants had lower respiration rates and a relative increase in alternative respiration. Furthermore, the decrease in CYTc severely affected the activity and the amount of Complex IV, without affecting Complexes I and III. Reactive oxygen species levels were reduced in these mutants, which showed induction of genes encoding antioxidant enzymes. Ascorbic acid levels were not affected, suggesting that a small amount of CYTc is enough to support its normal synthesis. We postulate that, in addition to its role as an e...Continue Reading

References

Mar 26, 1992·Nature·G T Babcock, M Wikström
Mar 25, 1991·Nucleic Acids Research·K EdwardsC Thompson
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·S GutierresR De Paepe
Mar 28, 1998·The Journal of Biological Chemistry·H BoumansJ A Berden
Oct 12, 1999·Plant Physiology·M W DaveyM C Van Montagu
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Nicholas SmirnoffFrank A Loewus
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Ian M Moller
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·L J SweetloveA H Millar
Jan 11, 2003·The Journal of Biological Chemistry·Antoni BarrientosAlexander Tzagoloff
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Oct 14, 2003·Plant Physiology·A Harvey MillarChristine H Foyer
Feb 14, 2004·Plant Physiology·Patricia Müller-MouléKrishna K Niyogi
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Greg C. Vanlerberghe, Lee McIntosh
Oct 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Etienne H MeyerGéraldine Bonnard
Jan 13, 2006·The Journal of Biological Chemistry·Stephanie SunderhausHans-Peter Braun
Sep 5, 2006·Trends in Plant Science·Georgette C BriggsChristian S Hardtke
Feb 27, 2007·Mitochondrion·David M Rhoads, Chalivendra C Subbaiah
Feb 27, 2007·The Journal of Biological Chemistry·Jesco HeinemeyerRoman Kouril
Apr 5, 2007·Nature Protocols·Ilka WittigHermann Schägger
Apr 21, 2007·Nature Protocols·Kelly M Gillespie, Elizabeth A Ainsworth
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Daniel H GonzalezEduardo F Mufarrege

❮ Previous
Next ❯

Citations

Apr 29, 2014·Mitochondrion·Pascal Touzet, Etienne H Meyer
Oct 6, 2015·Plant, Cell & Environment·Lucila GarciaDaniel H Gonzalez
Jan 2, 2016·Journal of Experimental Botany·Juan Pablo CórdobaEduardo Zabaleta
Jan 7, 2016·Frontiers in Plant Science·Ivan RadinIris Steinebrunner
Apr 12, 2013·The Plant Journal : for Cell and Molecular Biology·Cordelia BolleBernd Weisshaar
Sep 3, 2013·Biochimie·Catherine Colas des Francs-Small, Ian Small
Feb 1, 2018·The Plant Journal : for Cell and Molecular Biology·Sofía RaccaDaniel H Gonzalez
Mar 3, 2018·International Journal of Molecular Sciences·Natanael MansillaElina Welchen
Jul 11, 2018·PloS One·Habibullah Khan AchakzaiAbdul Kabir Khan Achakzai
Jul 30, 2018·Environmental Science and Pollution Research International·Xiao-Zhang YuYan-Hong Li
Apr 27, 2017·Frontiers in Plant Science·Islam MostafaSixue Chen
Feb 24, 2017·Frontiers in Plant Science·Jakob PetereitHolger Eubel
Apr 26, 2020·Mitochondrion·Hans-Peter Braun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.