Lack of evidence for an increase in interleukin-6 expression in adult murine bone, bone marrow, and marrow stromal cell cultures after ovariectomy

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
S J VargasJ A Lorenzo

Abstract

Interleukin-6 (IL-6) has been implicated as a mediator of postmenopausal bone loss. In vitro studies of bone and bone marrow cells have suggested that estrogen regulates bone turnover by controlling the production of IL-6, a potent stimulator of osteoclastogenesis and bone resorption. To investigate this hypothesis in an in vivo model, we examined the effect of ovariectomy or estrogen replacement on IL-6 mRNA and protein expression in adult mouse bone and bone marrow in vivo and in marrow stromal cell cultures. Eight-week-old CD-1 mice were sham-operated (SHAM), ovariectomized (OVX), or ovariectomized and subcutaneously implanted with 21-day slow-release pellets containing 10 micrograms of 17 beta-estradiol (O + E). Placebo pellets were implanted in the SHAM and OVX mice. Uterine weights at 1, 2, or 3 weeks after surgery were significantly decreased (68-76%) in OVX animals compared with SHAM or O + E. In mice sacrificed at 1 or 3 weeks after surgery, we found by nonquantitative reverse transcribed polymerase chain reaction (RT-PCR), that SHAM, OVX, and O + E calvariae (CALV) constitutively expressed IL-6 mRNA. In contrast, IL-6 mRNA was either barely detectable or absent in the tibia (TIB) and bone marrow (BM). In the mice sacr...Continue Reading

References

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Citations

Oct 28, 2011·Clinical Science·Masahiko MiharaMasashi Shiina
Feb 15, 2002·Endocrine Reviews·Johannes PfeilschifterHelmut Schatz
Mar 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Francisco A SylvesterGloria A Gronowicz
Oct 1, 1996·Nature Medicine·L G Raisz
Nov 25, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·A MarusicJ A Lorenzo
Mar 12, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R L Van BezooijenC W Löwik
Mar 10, 1998·Journal of Cellular Physiology·U SarmaA M Flanagan
Mar 13, 1999·Archives of Oral Biology·N Kawashima, P Stashenko

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