Lack of expression of dopamine D2 receptors in malignant melanoma: evidence for interaction of iodobenzofurans with melanin

Dermatology : International Journal for Clinical and Investigative Dermatology
R BöniG Westera

Abstract

(1) To compare scintigraphy using the new dopamine D2 receptor binding radioligand iodobenzofuran (IBF) versus whole-body positron emission tomography (PET) in demonstrating metastasizing melanoma, and (2) to determine, for the first time using a panel of histochemical techniques, whether the ability of D2 receptor binding radioligands to detect melanoma metastases is due to tumor-expressed D2 receptors. Seven patients with metastatic melanoma were examined using 123I-IBF scintigraphy. Findings were compared to the results of PET and metastasis histochemistry: D2 receptor mRNA assay (metastases: n = 5; melanoma cell lines: n = 4) using the reverse transcriptase polymerase chain reaction (RT-PCR) versus D2 receptor-transfected Chinese hamster ovary cell controls: in vitro 125I-IBF binding (n = 19), and immunohistochemical staining for dopamine D2 receptor protein (n = 19). IBF scintigraphy detected 2/10 melanoma metastases detected by PET (sensitivity 20%). No dopamine D2 receptor mRNA was found in melanoma cells using RT-PCR. The binding of 125I-IBF correlated with the amount of melanin present in the metastases; two amelanotic melanomas both failed to bind 125I-IBF. Immunohistochemical staining was negative in all metastases. ...Continue Reading

Citations

Sep 4, 2013·Journal of Dermatological Science·Noelia A MassariElena S Rivera
Feb 19, 2004·Cancer Biotherapy & Radiopharmaceuticals·Lisa BodeiDik J Kwekkeboom
Jan 25, 2006·Melanoma Research·Martin M Edreira, Oscar R Pozzi
Sep 24, 2004·Physiological Reviews·Andrzej SlominskiJacobo Wortsman

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