Lack of expression of the chondroitin sulphate proteoglycan neuron-glial antigen 2 on candidate stem cell populations in paediatric acute myeloid leukaemia/abn(11q23) and acute lymphoblastic leukaemia/t(4;11)

British Journal of Haematology
J NeudenbergerJosef Vormoor

Abstract

It has increasingly been acknowledged that only a few leukaemic cells possess the capability to renew themselves and that only these self-renewing leukaemic stem cells are able to initiate relapses. Therefore, these leukaemic stem cells should be the target cells for therapy and for minimal residual disease (MRD) detection. Because of its presence on blasts of 11q23-rearranged high-risk leukaemic patients, neuron-glial antigen 2 (NG2) is thought to be a valuable marker for detecting leukaemic stem cells. Six acute myeloid leukaemia (AML)/abn(11q23) and three acute lymphoblastic leukaemia (ALL)/t(4;11) samples were analysed by four-colour flow cytometry for NG2 expression on primitive cell populations. Candidate leukaemic cell populations were defined by the antigen profiles CD34+CD38- in AML and CD34+CD19-CD117+ in ALL. Surprisingly, in all patients these candidate stem cell populations were shown to lack expression of NG2. Instead, a correlation between the expression of the myeloid differentiation marker CD33 and increasing levels of NG2 on maturing cells could be demonstrated. Similarly, in ALL patients CD34+CD19+ cells showed a higher expression of NG2 mRNA compared with CD34+CD19-. Thus, NG2 appears to be upregulated with ...Continue Reading

References

Oct 10, 1998·Journal of Cellular Physiology·M A BurgW B Stallcup
Dec 5, 2003·Transfusion Medicine·A Blair, D H Pamphilon
Aug 16, 2005·British Journal of Haematology·Dominique Bonnet

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Citations

Oct 4, 2011·Cellular and Molecular Life Sciences : CMLS·Elena GarusiRoberto Perris
Oct 29, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna SmirkinJunya Tanaka
Apr 16, 2011·Leukemia Research·Pablo Menendez, Clara Bueno
Apr 17, 2007·Experimental and Molecular Pathology·Mohamed Abdelhaleem
Jul 6, 2010·Developmental Biology·Feng-Ju HuangWilliam B Stallcup
Mar 4, 2009·Biochemical and Biophysical Research Communications·Hiroko NishidaChikao Morimoto
Jun 15, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hiroaki MatsumotoJunya Tanaka
Jul 18, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Maria Serena BenassiRoberto Perris

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