Lack of FSH support enhances LIF-STAT3 signaling in granulosa cells of atretic follicles in cattle

Reproduction : the Official Journal of the Society for the Study of Fertility
Gustavo Freitas IlhaRaj Duggavathi

Abstract

Subordinate follicles (SFs) of bovine follicular waves undergo atresia due to declining FSH concentrations; however, the signalling mechanisms have not been fully deciphered. We used an FSH-induced co-dominance model to determine the effect of FSH on signalling pathways in granulosa cells of the second-largest follicles (SF in control cows and co-dominant follicle (co-DF2) in FSH-treated cows). The SF was smaller than DF in control cows while diameters of co-DF1 and co-DF2 in FSH-treated cows were similar. The presence of cleaved CASP3 protein confirmed that granulosa cells of SFs, but not of DFs and co-DFs, were apoptotic. To determine the effect of FSH on molecular characteristics of the second-largest follicles, we generated relative variables for the second largest follicle in each cow. For this, variables of SF or co-DF2 were divided by the variables of the largest follicle DF or co-DF1 in each cow. There was higher transcript abundance of MAPK1/3 and AKT1/2/3 but lower abundance of phosphorylated MAPK3/1 in SF than co-DF2 granulosa cells. Abundance of mRNA and phosphorylated protein of STAT3 was higher in granulosa cells of control SF than FSH-treated co-DF2. SF granulosa cells had higher levels of LIFR and IL6ST transcri...Continue Reading

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Citations

Jan 28, 2016·Reproduction in Domestic Animals = Zuchthygiene·G F IlhaP B D Gonçalves
Dec 22, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Rui-Song YeYong-Liang Zhang
Nov 22, 2020·Journal of Ovarian Research·Yao JiangXiaolong Yuan

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