Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean

Molecular Plant-microbe Interactions : MPMI
Juan Ignacio QuelasAníbal R Lodeiro

Abstract

Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from Bradyrhizobium japonicum are important for infection and nodulation of soybean (Glycine max), although their roles are not completely understood. To better understand this, we constructed mutants in B. japonicum USDA 110 impaired in galactose or galacturonic acid incorporation into the EPS without affecting the LPS. The derivative LP 3010 had a deletion of lspL-ugdH and produced EPS without galacturonic acid whereas LP 3013, with an insertion in exoB, produced EPS without galactose. In addition, the strain LP 3017, with both mutations, had EPS devoid of both galactosides. The missing galactosides were not replaced by other sugars. The defects in EPS had different consequences. LP 3010 formed biofilms and nodulated but was defective in competitiveness for nodulation; and, inside nodules, the peribacteroid membranes tended to fuse, leading to the merging of symbiosomes. Meanwhile, LP 3013 and LP 3017 were unable to form biofilms and produced empty pseudonodules but exoB suppressor mutants were obtained when LP 3013 plant inoculation was supplemented with wild-type EPS. Similar phenotypes were observed with all these mutants in G. soja. Therefore, the lack of each galactosi...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jan 1, 1992·Annual Review of Microbiology·J A Leigh, D L Coplin
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L BattistiJ A Leigh
Apr 1, 1990·Journal of Bacteriology·M CarrionR W Carlson
Jan 1, 1987·Journal of Bacteriology·V PuvanesarajahG Stacey
Jun 1, 1985·Journal of Bacteriology·K A VandenboschE H Newcomb
Aug 1, 1994·Trends in Microbiology·E L Kannenberg, N J Brewin
Aug 25, 1999·Current Opinion in Plant Biology·A M Hirsch
Aug 31, 1999·Planta·E FedorovaD A Day
Mar 22, 2000·Plant Science : an International Journal of Experimental Plant Biology·A R LodeiroG Favelukes
Jun 30, 2000·Molecular Plant-microbe Interactions : MPMI·M CermolaE J Patriarca
Feb 7, 2001·Applied and Environmental Microbiology·H A Louch, K J Miller
May 3, 2001·Molecular Plant-microbe Interactions : MPMI·L BolañosI Bonilla
May 16, 2002·Environmental Microbiology·Silvina L López-GarcíaAníbal R Lodeiro
Feb 25, 2003·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takakazu KanekoSatoshi Tabata
Mar 26, 2003·European Journal of Biochemistry·Nicolas FraysseVéréna Poinsot
Jul 1, 1961·Journal of Bacteriology·J H LAW, R A SLEPECKY
Apr 7, 2004·International Review of Cytology·Eduardo J PatriarcaMaurizio Iaccarino
May 27, 2005·Molecular Plant-microbe Interactions : MPMI·Alejandra L D'AntuonoViviana C Lepek
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Maribel ParadaJosé E Ruiz-Sainz
Apr 25, 2006·FEMS Microbiology Ecology·Nancy A FujishigeAnn M Hirsch
Jul 17, 2007·Nature Reviews. Microbiology·Kathryn M JonesGraham C Walker

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Citations

May 15, 2013·Journal of Bacteriology·J Ignacio QuelasAníbal R Lodeiro
Dec 17, 2011·International Journal of Molecular Sciences·Monika Janczarek
Nov 6, 2012·Research in Microbiology·Julieta Mariana CovelliAníbal Roberto Lodeiro
Sep 14, 2015·Revista Argentina de microbiología·Aníbal R Lodeiro
Apr 8, 2011·FEMS Microbiology Letters·Maria Julia AlthabegoitiAníbal R Lodeiro
May 24, 2016·International Journal of Molecular Sciences·Francisco J López-BaenaJosé M Vinardell
Dec 19, 2014·PloS One·Dulce N Rodríguez-NavarroJosé M Vinardell
May 24, 2017·Journal of Bacteriology·Elías J MongiardiniAníbal R Lodeiro
May 22, 2016·Applied and Environmental Microbiology·J I QuelasA R Lodeiro
Sep 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Rachel M WheatleyPhilip S Poole

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