Lack of glyoxylate shunt dysregulates iron homeostasis in Pseudomonas aeruginosa

Microbiology
Sunhee HaWoojun Park

Abstract

The aceA and glcB genes, encoding isocitrate lyase (ICL) and malate synthase, respectively, are not in an operon in many bacteria, including Pseudomonas aeruginosa, unlike in Escherichia coli. Here, we show that expression of aceA in P. aeruginosa is specifically upregulated under H2O2-induced oxidative stress and under iron-limiting conditions. In contrast, the addition of exogenous redox active compounds or antibiotics increases the expression of glcB. The transcriptional start sites of aceA under iron-limiting conditions and in the presence of iron were found to be identical by 5' RACE. Interestingly, the enzymatic activities of ICL and isocitrate dehydrogenase had opposite responses under different iron conditions, suggesting that the glyoxylate shunt (GS) might be important under iron-limiting conditions. Remarkably, the intracellular iron concentration was lower while the iron demand was higher in the GS-activated cells growing on acetate compared to cells growing on glucose. Absence of GS dysregulated iron homeostasis led to changes in the cellular iron pool, with higher intracellular chelatable iron levels. In addition, GS mutants were found to have higher cytochrome c oxidase activity on iron-supplemented agar plates o...Continue Reading

References

Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P VanniB A McFadden
Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
Jun 1, 1968·European Journal of Biochemistry·E Vanderwinkel, M De Vlieghere
Jan 1, 1981·Molecular & General Genetics : MGG·M E Armengod
Oct 1, 1981·Journal of Bacteriology·S R Maloy, W D Nunn
Feb 1, 1996·Infection and Immunity·J M MeyerI A Holder
Aug 1, 1996·Journal of Bacteriology·L GuiD C LaPorte
Jan 28, 1999·Journal of Bacteriology·J F ProstJ C Cortay
Mar 30, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F PetratH de Groot
Mar 24, 2000·Archives of Biochemistry and Biophysics·F PetratU Rauen
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric Massé, Susan Gottesman
May 25, 2002·Journal of Bacteriology·David G KehresMichael E Maguire
Dec 21, 2002·Molecular Microbiology·Kaneyoshi Yamamoto, Akira Ishihama
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Feb 7, 2004·Molecular Microbiology·James C Comolli, Timothy J Donohue
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paula J WildermanMichael L Vasil
May 17, 2005·Nature Medicine·Ernesto J Muñoz-Elías, John D McKinney
Jun 7, 2005·Journal of Bacteriology·Brigid M DavisMatthew K Waldor
Jul 21, 2005·Journal of Bacteriology·Kelli L PalmerMarvin Whiteley
May 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eric E SmithMaynard V Olson
Aug 29, 2006·PLoS Pathogens·David B FriedmanEric P Skaar
Dec 23, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Michael L Vasil
Apr 22, 2008·The Journal of Biological Chemistry·Amanda G OglesbyMichael L Vasil
Jan 24, 2009·Microbial Pathogenesis·Thomas F DuceyDavid W Dyer
Jun 6, 2009·FEMS Microbiology Letters·Young Wook ChoiNa Gyong Lee
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Francesco ImperiPaolo Visca
Nov 26, 2009·Environmental Microbiology·Takuro KawakamiHiroyuki Arai

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Citations

Jun 13, 2019·Microbial Biotechnology·Stephen K DolanMartin Welch
Apr 2, 2020·Frontiers in Immunology·Sebastián A RiquelmeAlice Prince
Jan 5, 2020·Applied Microbiology and Biotechnology·Bora ShinWoojun Park
Feb 26, 2021·Metabolism: Clinical and Experimental·Christopher AugerMarc G Jeschke
Aug 29, 2021·Pathogens·Sebastián A Riquelme, Alice Prince

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