Lack of GVHD across classical, single minor histocompatibiliTy (miH) locus barriers in mice

Transplantation
B R BlazarD A Vallera

Abstract

To determine whether a disparity at a single miH genetic loci are sufficient to generate GVHD in mice, we focused on well-known genetic alleleic differences at the miH gene loci, H3 and H4. For H3 congenic GVHD studies, C57BL/10 (H2b) mice were used as recipients of miH-disparate B10.LP-H3b donor cells. For H4 congenic GVHD studies, C57BL/10 were used as recipients for miH-disparate B10.129 (21M)-H4b. To overcome the low frequency of miH-reactive CTLs in naive mice, multiple immunizations of the donor strains with host lymphohematopoietic cells were used. Peripheral blood cells from immunized mice were shown to have potent CTL activity against their respective host-type stimulator cells when analyzed 1 week prior to obtaining donor splenocytes for GVHD induction. Lethally irradiated C57BL/6 recipients of either 50 X 10(6) donor B10.LP-H3b or B10.129 (21M)-H4b splenocytes did not develop acute or chronic GVHD as assessed by monitoring the animals for survival, weight loss, splenic flow cytometry, and histological examination of skin, liver, colon, and lung in long-term survivors. Engraftment was documented in long-term chimeras in both strain combinations by using the post-BMT cells as alloantigen targets for cloned CTL lines sp...Continue Reading

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Citations

Mar 24, 1998·Immunology Today·C PerreaultC Fortin
Apr 18, 2007·Nature Reviews. Immunology·Warren D Shlomchik
Feb 24, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Christine G DiRienzoRobert Korngold
Apr 13, 2004·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Nelson J Chao
Jul 8, 2011·Blood·Krystel VincentClaude Perreault
Sep 16, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stacey L FanningThea M Friedman
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel H KaplanWarren D Shlomchik
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E Y ChoiD C Roopenian

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