Lack of malate valve capacities lead to improved N-assimilation and growth in transgenic A. thaliana plants

Plant Signaling & Behavior
Jennifer Selinski, Renate Scheibe

Abstract

In this study we analyzed the relationship between malate valve capacities, N-assimilation, and energy metabolism. We used transgenic plants either lacking the chloroplast NADP-dependent malate dehydrogenase or mutants with a decreased transcript level of the plastid-localized NAD-dependent malate dehydrogenase. Plants were grown on nitrate or ammonium, respectively, as the sole N-source and transcripts were analyzed by qRT-PCR. We could show that the lack of malate valve capacities enhances N-assimilation and plastidial glycolysis by increasing transcript levels of Fd-GOGATs or NADH-GOGAT and plastidic NAD-GAPDHs (GapCps), respectively. Based on our results, we conclude that the lack of malate valve capacities is balanced by an increase of the activity of plastid-localized glycolysis in order to cover the high demand for plastidial ATP, stressing the importance of the plastids for energy metabolism in plant cells.

References

Oct 17, 1998·The Journal of Biological Chemistry·M BerkemeyerO Ocheretina
Feb 15, 2002·The Plant Journal : for Cell and Molecular Biology·Muriel LancienGloria M Coruzzi
Jul 23, 2003·Bioinformatics·Perikles Simon
Mar 23, 2004·Physiologia Plantarum·Renate Scheibe

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Citations

Sep 6, 2020·BMC Plant Biology·Magda GrabsztunowiczPaula Mulo
Jun 23, 2018·Plant Biology·J Selinski, R Scheibe
Jun 20, 2018·The Plant Journal : for Cell and Molecular Biology·Shin-Nosuke HashidaMaki Kawai-Yamada
Dec 9, 2014·Plant Signaling & Behavior·Jennifer Selinski, Renate Scheibe

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Methods Mentioned

BETA
PCR

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