PMID: 7538424Jan 1, 1995Paper

Lack of role for nitric oxide (NO) in the selective destabilization of endothelial NO synthase mRNA by tumor necrosis factor-alpha

Arteriosclerosis, Thrombosis, and Vascular Biology
F MohamedD J Stewart

Abstract

The constitutive expression of endothelial nitric oxide (NO) synthase (cNOS) is essential for the physiological regulation of vascular tone and structure. The mechanism of downregulation of steady state cNOS mRNA in human umbilical vein endothelial cells exposed to tumor necrosis factor-alpha (TNF-alpha) was investigated by using Northern blot analysis of total cellular RNA. TNF-alpha produced a dose- and time-dependent decrease in cNOS mRNA expression that was near maximal at 10 U/mL and 6 hours of exposure, respectively. In contrast, steady state expression of endothelin-1 and plasminogen activator inhibitor-1 (PAI-1) mRNA was upregulated by TNF-alpha. The pharmacological generation of NO using sodium nitroprusside (10 mumol/L) and S-nitroso-acetylpenicillamine (100 to 400 mumol/L) had no effect on cNOS mRNA levels, and TNF-alpha-induced downregulation of cNOS was not prevented by coincubation with the inhibitors of NO synthesis N omega-nitro-L-arginine methyl ester (1 mmol/L) and NG-monomethyl L-arginine (10 mmol/L). Under control conditions, cNOS and PAI-1 mRNA were stable after treatment with actinomycin D for periods greater than 24 hours, whereas endothelin-1 message was rapidly degraded (half-life, < 1 hour). Pretreatme...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinS H Snyder
Oct 1, 1991·The American Journal of Physiology·S LamasP A Marsden
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M W RadomskiS Moncada
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D J StuehrC F Nathan
Jan 1, 1991·Advances in Experimental Medicine and Biology·B M EwensteinK A Birch
May 1, 1991·The American Journal of Physiology·J StadlerR L Simmons
Jul 26, 1990·The New England Journal of Medicine·B LevineM Packer
Sep 1, 1988·Journal of the American College of Cardiology·T M GriffithA H Henderson
Oct 23, 1986·The New England Journal of Medicine·P L LudmerP Ganz
Dec 1, 1986·The Journal of Clinical Investigation·D GinsburgT D Gelehrter
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Aug 13, 1993·Cell·A B Sachs

❮ Previous
Next ❯

Citations

Jul 25, 1997·Atherosclerosis·G R UpchurchJ Loscalzo
Jan 1, 1996·Biochimie·J P PasquetV Ullrich
Apr 1, 1998·Trends in Cardiovascular Medicine·R C Leboeuf, S A Schreyer
Jan 16, 2010·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Sébastien CzernichowBernard I Levy
Feb 15, 2000·European Journal of Biochemistry·M SwiatkowskaC S Cierniewski
Jun 2, 1998·Kidney International·B J Ballermann
Mar 29, 2011·Acta Physiologica·M Tesauro, C Cardillo
Nov 7, 2007·European Journal of Endocrinology·Juliet EvansJulia H Goedecke
Nov 2, 2013·International Journal of Molecular Sciences·Dario PitoccoCarmine Cardillo
Mar 1, 2006·Expert Review of Endocrinology & Metabolism·Renate T de JonghCoen DA Stehouwer
May 15, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Bonnie L GoodwinDuane C Eichler
Sep 8, 2011·British Journal of Pharmacology·Umberto CampiaCarmine Cardillo
Apr 26, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Nicola J Menzies-GowJ Elliott
Dec 17, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ingrid Fleming, Rudi Busse
Apr 24, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anna CsiszarGabor Kaley
Jun 2, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Manjula PandeyFahumiya Samad
Jan 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arthur C LiuJonathan C Choy
Aug 1, 1996·Critical Care Medicine·H G Bone, D L Traber
Jun 2, 2006·American Journal of Physiology. Cell Physiology·Charles D Searles
Mar 20, 2018·Journal of UOEH·Keiichi TorimotoYoshiya Tanaka
May 7, 2002·American Journal of Hypertension·Shota SasakiKazuaki Chayama
Aug 29, 2012·The Journal of Obstetrics and Gynaecology Research·Na-Jung OhSe-Ryun Kim
Mar 5, 2009·American Journal of Physiology. Endocrinology and Metabolism·Do-Eun LeeJohn S Yudkin
Sep 27, 2000·Journal of the American Society of Nephrology : JASN·María M ArrieroAntonio López-Farré

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.