Abstract
In order to identify a specific recombinant antigen of Leishmania donovani with potential use for diagnosis, a cDNA library was constructed in lambda ZAP II expression vector. On screening the cDNA library using pooled sera from Indian patients with kala azar, 20 antibody reactive clones were identified. These were subcloned into pBluescript phagemid by an in vivo excision procedure. The molecular weights of the expressed recombinant proteins varied from 15 to 70 kDa and the cDNA insert sizes varied from 0.5 kb to the largest size of approximately 2.0 kb which was designated as the E2b clone. The nucleotide sequencing revealed that 50% of the clones had sequence homology to the heat shock protein gene of L. donovani. The serological studies conducted with the kala azar positive sera and sera from healthy laboratory workers using the recombinant protein from the E2b clone and having sequence homology to Ldhsp 70, indicated that although all the kala azar sera was positive, 12 of 20 healthy individuals also showed antibodies against the recombinant hsp70, indicating that this antigen is not suitable for serological diagnosis of kala azar.
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 27, 1979·Biochemistry·J M ChirgwinW J Rutter
Jul 1, 1992·Infection and Immunity·G R WallaceJ M Kelly
Feb 1, 1992·Journal of Clinical Microbiology·C R de AndradeK Otsu
May 1, 1990·Infection and Immunity·N KumarH Zheng
Nov 1, 1991·Lupus·V DhillonD Isenberg
Oct 1, 1989·Infection and Immunity·P C Melby, D L Sacks
Apr 2, 1987·Journal of Immunological Methods·C Abou-ZeidG A Rook
Oct 25, 1988·Nucleic Acids Research·M G LeeL H Van der Ploeg
Aug 1, 1986·Journal of Neuroimmunology·H Reiber, A J Suckling
Jun 21, 1985·Science·L H Van der PloegC R Cantor
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Aug 15, 1970·Nature·U K Laemmli
Dec 14, 1984·Biochemical and Biophysical Research Communications·K W HunterE G Hayunga
Apr 1, 1993·Clinical and Experimental Immunology·I Kindås-MüggeJ S Smolen
Jan 1, 1964·Advances in Parasitology·S ADLER
Citations
Sep 25, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sunil K AroraN L Sharma
Apr 8, 1999·Clinical and Experimental Immunology·H KakeyaS Kohno
Nov 5, 2002·Clinical and Diagnostic Laboratory Immunology·Rosângela Barbosa-De-DeusCarlos Alberto Pereira Tavares
Jun 23, 2006·Infection and Immunity·Yasuyuki GotoSteven G Reed
Dec 7, 2011·The American Journal of Tropical Medicine and Hygiene·Geraldo G S OliveiraOsvaldo P de Melo Neto
Feb 17, 2005·Experimental Parasitology·Sunil K AroraS Mujtaba
Jun 27, 1998·Experimental Parasitology·S L EllisD M Dwyer
Jun 7, 2011·Experimental Parasitology·Shet MasihRakesh K Vasishta
Feb 20, 2007·Memórias do Instituto Oswaldo Cruz·Edward José de OliveiraMário Hiroyuki Hirata
Sep 10, 2002·Memórias do Instituto Oswaldo Cruz·Herminia Y KanamuraRaymond T Damian
Dec 1, 2006·Dermatology : International Journal for Clinical and Investigative Dermatology·Teoman Zafer Apan, Sedat Kaygusuz
Jun 10, 1998·Immunology and Cell Biology·S K AroraS Sehgal
Dec 13, 2016·Transactions of the Royal Society of Tropical Medicine and Hygiene·Anuradha Ratna, Sunil K Arora
Aug 13, 2003·Parasite Immunology·E OrtonaA Siracusano