PMID: 8915423Nov 1, 1996Paper

Lack of serum antibodies against glycoprotein E in pseudorabies virus-immune pigs infected with wild-type virus

American Journal of Veterinary Research
L JacobsA T Bianchi

Abstract

To examine whether pigs with solid immunity against pseudorabies virus (PRV) could harbor latent infection with wild-type PRV without developing antibodies against glycoprotein E (gE), which is used as a marker protein to differentiate pigs that have been vaccinated from pigs infected with wild-type PRV. Specific-pathogen-free pigs that were seronegative for antibodies to PRV. Oropharyngeal swab specimens were collected, and virus content was measured, Serum samples were obtained 1 week before and 2 and 4 weeks after challenge exposure. Four weeks after challenge exposure, pigs were slaughtered; various tissues were collected for virus isolation, and DNA was analyzed by polymerase chain reaction. Of the intranasally inoculated pigs, only 1 pig shed virus (for 1 day) but none developed antibodies directed against gE. Of the inoculated IM pigs, 3 pigs shed virus (for several days) and 5 developed antibodies directed against gE. One pig did not shed virus and did not develop detectable gE antibodies. However, variable amounts of wild-type virus DNA were detected in various tissues. Immunized pigs can be infected with wild-type PRV without being detected by the gE-specific ELISA, which is routinely used to discriminate between infe...Continue Reading

Related Concepts

Glycoprotein E, Suid herpesvirus 1
Antibodies, Viral
Antibody Formation
Gene Amplification
Pseudorabies
Herpesvirus 1, Suid
Viral Envelope Proteins gp70
Nested Polymerase Chain Reaction

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