Lacrimal Gland Myoepithelial Cells Are Altered in a Mouse Model of Dry Eye Disease.

The American Journal of Pathology
Laura García-PosadasDarlene A Dartt

Abstract

The purpose of this study was to determine the pathogenic changes that occur in myoepithelial cells (MECs) from lacrimal glands of a mouse model of Sjögren syndrome. MECs were cultured from lacrimal glands of C57BL/6J [wild type (WT)] and thrombospondin 1 null (TSP1-/-, alias Thbs1-/-) mice and from mice expressing α-smooth muscle actin-green fluorescent protein that labels MECs. MECs were stimulated with cholinergic and α1-adrenergic agonists, vasoactive intestinal peptide (VIP), and the purinergic agonists ATP and UTP. Then intracellular [Ca2+] was measured using fura-2, and contraction was observed using live cell imaging. Expression of purinergic receptors was determined by Western blot analysis, and mRNA expression was analyzed by microarray. The increase in intracellular [Ca2+]I with VIP and UTP was significantly smaller in MECs from TSP1-/- compared with WT mice. Cholinergic agonists, ATP, and UTP stimulated contraction in MECs, although contraction of MECs from TSP1-/- mice was reduced compared with WT mice. The amount of purinergic receptors P2Y1, P2Y11, and P2Y13 was significantly decreased in MECs from TSP1-/- compared with WT mice, whereas several extracellular matrix and inflammation genes were up-regulated in MECs...Continue Reading

References

Jun 30, 2006·Journal of Mammary Gland Biology and Neoplasia·Thorarinn GudjonssonMina J Bissell
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Apr 21, 2009·Progress in Retinal and Eye Research·Darlene A Dartt
Aug 25, 2009·The American Journal of Pathology·Bruce TurpieSharmila Masli
Sep 3, 2009·Cornea·Morgan L MassingalePenny A Asbell
Mar 23, 2011·Investigative Ophthalmology & Visual Science·Robin R HodgesDarlene A Dartt
Nov 1, 2011·Investigative Ophthalmology & Visual Science·Kaori OhtomoDarlene A Dartt
Nov 30, 2011·Developmental Biology·Nate WeymouthDon C Rockey
Mar 20, 2012·Investigative Ophthalmology & Visual Science·Marie A ShatosDarlene A Dartt
Dec 22, 2015·Current Molecular Biology Reports·Helen P Makarenkova, Darlene A Dartt
Oct 19, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Robin R Hodges, Darlene A Dartt
Jul 25, 2017·The Ocular Surface·Carlos BelmonteJames S Wolffsohn
Oct 20, 2018·International Journal of Molecular Sciences·Yukako TatematsuDarlene A Dartt

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Citations

Dec 4, 2020·International Journal of Molecular Sciences·Sharmila Masli, Darlene A Dartt
Apr 23, 2021·Stem Cell Research & Therapy·Sang Yun JeongJongman Yoo
Sep 22, 2021·Investigative Ophthalmology & Visual Science·Martin DankisMichael Winder

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