Lactase gene promoter fragments mediate differential spatial and temporal expression patterns in transgenic mice

DNA and Cell Biology
Zhi WangEric Sibley

Abstract

Lactase gene expression is spatiotemporally regulated during mammalian gut development. We hypothesize that distinct DNA control regions specify appropriate spatial and temporal patterning of lactase gene expression. In order to define regions of the lactase promoter involved in mediating intestine-specific and spatiotemporal restricted expression, transgenic mice harboring 100 bp, 1.3- and 2.0- kb fragments of the 5' flanking region of the rat lactase gene cloned upstream of a luciferase reporter were characterized. The 100-bp lactase promoter-reporter transgenic mouse line expressed maximal luciferase activity in the intestine with a posterior shift in spatial restriction and ectopic expression in the stomach and lung. The temporal pattern of expression mediated by the 1.3-kb promoter?reporter transgene increases with postnatal maturation in contrast with the postnatal decline mediated by the 2.0-kb promoter-reporter transgene and the endogenous lactase gene. The differential transgene expression patterns mediated by the lactase promoter fragments suggests that intestine-specific spatial and temporal control elements reside in distinct regions of the DNA sequences upstream of the lactase gene transcription start-site.

References

Apr 26, 1995·Biochemical and Biophysical Research Communications·M VerhaveR J Grand
Jul 12, 1996·The Journal of Biological Chemistry·B RomagnoloC Perret
Mar 30, 2001·The Journal of Biological Chemistry·M R DusingD A Wiginton
Jan 29, 2002·The Journal of Biological Chemistry·So Young LeeEric Sibley

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Citations

Nov 27, 2008·Human Genetics·Catherine J E IngramDallas M Swallow
Sep 3, 2013·American Journal of Human Genetics·Bryony L JonesDallas M Swallow
Jun 9, 2016·Nature Structural & Molecular Biology·Dallas M Swallow, Jesper T Troelsen
Sep 22, 2017·Diseases·Bodo C Melnik, Gerd Schmitz

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